This research aimed at analysing the protective effect of quercetin against the nephrotoxicity of acrylamide (AA) using metabonomics technology. Rats were randomly divided into six groups, including the control group, AA-treated group (5 mg per kg bw per d), two dosages of quercetin-treated groups (10 and 50 mg per kg bw per d) and two dosages of quercetin plus AA-treated groups. Quercetin and AA were administered to rats daily via gavage and drinking water, respectively. After a 16 week treatment, the kidneys were collected for metabonomics analysis. A total of 14 metabolites were identified in positive and negative modes via the metabonomics profiles of kidney tissues. Compared with the control group, the intensities of uric acid, 6,8-dihydroxypurine, hypoxanthine, guanosine, L-phenylalanine, succinyladenosine, tryptophan, lysophosphatidylcholines (LPC(16 : 0), LPC(18 : 1(9z)), LPC(18 : 2(9z,12z))) and inosine in the AA-treated group were significantly increased (p < 0.01); and the intensities of L-acetylcarnitine, 2-phenylacetamide and pantothenic acid in the AA-treated group were decreased (p < 0.01). However, the intensities of the previously mentioned metabolites had opposite trends in the high-dose quercetin (50 mg per kg bw per d) plus AA-treated groups (p < 0.01 or p < 0.05). The results showed that quercetin exerted protective effects on AA-induced nephrotoxicity by enhancing the antioxidant defence system of the body and regulating phospholipid, energy and amino acid metabolism.

中文翻译：

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槲皮素对大鼠丙烯酰胺肾毒性的代谢组学分析†

这项研究旨在使用代谢组学技术分析槲皮素对丙烯酰胺（AA）的肾毒性的保护作用。将大鼠随机分为六组，包括对照组，AA治疗组（5 mg / kg bw / d），两剂槲皮素治疗组（10和50 mg / kg bw / d）和两剂槲皮素加上AA治疗组。每天分别通过管饲法和饮用水将槲皮素和AA给药于大鼠。治疗16周后，收集肾脏进行代谢组学分析。通过肾脏组织的代谢组学分析，共鉴定出14种代谢物呈阳性和阴性模式。与对照组相比，尿酸，6,8-二羟基嘌呤，次黄嘌呤，鸟苷，AA治疗组的L-苯丙氨酸，琥珀酰腺苷，色氨酸，溶血磷脂酰胆碱（LPC（16：0），LPC（18：1（9z）），LPC（18：2（9z，12z）））和肌苷显着增加（p <0.01）; AA治疗组的L-乙酰肉碱，2-苯基乙酰胺和泛酸的强度降低（p <0.01）。但是，在大剂量槲皮素（每公斤体重每天50毫克）和AA处理组中，前述代谢产物的强度具有相反的趋势（p <0.01或p<0.05）。结果表明，槲皮素通过增强机体的抗氧化防御系统并调节磷脂，能量和氨基酸代谢，对AA诱导的肾毒性发挥保护作用。