Abstract A facile, sensitive and selective electrochemical biosensor for the detection of dopamine (DA) released from nicotine-stimulated PC12 cells was fabricated by using a poly-celestine blue (CB) modified glassy carbon electrode (GCE). The poly-CB film was prepared by controlled electropolymerization and has about 5 nm thick on the GCE surface. In the optimal experimental condition, the poly-CB modified GCE showed excellent electrocatalytic activity toward the oxidation of DA. The response signal obtained by differential pulse voltammetry displayed a good linear relationship between the current response signal and the DA concentrations in range from 10 nmol L−1 to 0.7 μmol L−1 and 1 μmol L−1 to 10 μmol L−1 with a detection limit of 1.2 nmol L−1 (S/N = 3) and sensitivity of 17.0134 μA cm2 μM−1. In addition, the poly-CB modified GCE has successfully been applied to determine nicotine-induced dopamine released from PC12 cells with satisfactory recovery. The developed method held great potential for the detection of dopamine in biological samples and would bring great convenience to the diagnosis of diseases associated with deficiency in dopamine.

中文翻译：

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一种用于灵敏检测 PC12 细胞分泌的尼古丁诱导的多巴胺的电化学生物传感器

摘要 使用聚天青石蓝 (CB) 修饰的玻碳电极 (GCE) 制备了一种简便、灵敏且选择性的电化学生物传感器，用于检测尼古丁刺激的 PC12 细胞释放的多巴胺 (DA)。聚 CB 膜是通过受控电聚合制备的，并且在 GCE 表面上具有约 5 nm 厚。在最佳实验条件下，聚CB修饰的GCE对DA的氧化表现出优异的电催化活性。差分脉冲伏安法获得的响应信号显示电流响应信号与 DA 浓度之间存在良好的线性关系，范围为 10 nmol L-1 至 0.7 μmol L-1 和 1 μmol L-1 至 10 μmol L-1，其中检测限为 1.2 nmol L-1 (S/N = 3)，灵敏度为 17.0134 μA cm2 μM-1。此外，聚 CB 修饰的 GCE 已成功应用于测定尼古丁诱导的 PC12 细胞释放的多巴胺，回收率令人满意。所开发的方法在生物样品中多巴胺的检测方面具有很大的潜力，将为多巴胺缺乏相关疾病的诊断带来极大的便利。