This study aimed to systematically investigate the effect of organic loading on granule diameters, and on the composition of extracellular polymeric substances (EPS) in granules in various size-fractions at the beginning and end of the cycle of granular sludge sequencing batch reactor (GSBR). The organic loadings were 0.78 kg COD/(m³·d) (GSBR1), 1.16 kg COD/(m³·d) (GSBR2) and 1.53 kg COD/(m³·d) (GSBR3). Granules with a diameter of 0.5–1 mm had the most stable EPS content and composition. The smallest granules had the largest amount of bound EPS. The amount of loosely-bound EPS increased as granule diameters decreased; it was lowest in the famine phase at end of the cycle. The proteins/polysaccharides ratio decreased below 1 only in soluble EPS in the famine period. In GSBR1, granules with a diameter <0.5 mm predominated, and the increase in soluble EPS at end of the cycle was most substantial resulting in the lowest COD removal.

这项研究旨在系统地研究颗粒污泥测序间歇反应器（GSBR）循环开始和结束时有机负荷对颗粒直径以及颗粒大小不同的颗粒中细胞外聚合物质（EPS）组成的影响。 。有机负荷为0.78 kg COD /（m ³ ·d）（GSBR1），1.16 kg COD /（m ³ ·d）（GSBR2）和1.53 kg COD /（m ³ ·d）（1.5）·d）（GSBR3）。直径为0.5–1 mm的颗粒具有最稳定的EPS含量和组成。最小的颗粒具有最大的结合EPS量。随着颗粒直径的减小，松散结合的EPS的数量增加。在周期结束时饥荒阶段最低。在饥荒时期，仅可溶性EPS中的蛋白质/多糖比降低至1以下。在GSBR1中，直径小于0.5毫米的颗粒占主导地位，并且在循环结束时可溶性EPS的增加最为显着，导致最低的COD去除率。