This study investigated the interrelations between hydrogen synthesis and Photosystem I electron transport rate in . The fluorescence of both photosystems (PS I and PS II) was monitored using a Dual Pulse Amplitude Modulated (PAM) Fluorometer. Hydrogen synthesis was induced by eliminating sulphur from the growth media (TAP-S). Multiple physiological parameters [rETR, Y (I), Y (II), NPQ, α, F/F and YI:YII] were recorded using the Dual PAM and correlated to hydrogen produced. There was a 66% increase in Photosystem I rETR during hydrogen production. A significant direct correlation existed between PS 1 rETR and hydrogen evolution values over the ten-day period (r = 0.895, < 0.01) indicating that PS I can be considered as a driver of H production. Significant correlations between rETR of PS I and H evolution suggest a novel physiological indicator to monitor H production during the three critical phases identified in this study.

中文翻译：

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光系统 I 荧光作为莱茵衣藻产氢的生理指标


本研究调查了氢合成与光系统 I 电子传输速率之间的相互关系。使用双脉冲幅度调制 (PAM) 荧光计监测两个光系统（PS I 和 PS II）的荧光。通过消除生长培养基（TAP-S）中的硫来诱导氢合成。使用 Dual PAM 记录多个生理参数 [rETR、Y (I)、Y (II)、NPQ、α、F/F 和 YI:YII]，并与产生的氢气相关联。在氢气生产过程中，光系统 I rETR 增加了 66%。十天期间 PS 1 rETR 和析氢值之间存在显着的直接相关性（r = 0.895，< 0.01），表明 PS I 可以被视为 H 产生的驱动因素。 PS I 的 rETR 与 H 进化之间的显着相关性表明，有一种新的生理指标可以监测本研究中确定的三个关键阶段的 H 产生。