Lipid extraction is a key step of biodiesel production from microalgae, however, the application of traditional extraction methods was limited due to the difficulties of cell disruption as well as solvent toxicity. In this work, pretreatment method using pulsed electric field (PEF), was primarily applied to lipid extraction process from microalgae Chlorella pyrenoidosa grown in wastewater. After the pretreatment with PEF, the yields of fatty acid methyl esters from C.pyrenoidosa was 12.0% higher than traditional pretreatment with ultrasonic. The results indicated that PEF was an effective method for cell disruption. Fluorescence staining and scanning electron microscopy showed that the integrity of the cell membrane of microalgae was damaged under pulsed electric field, which enhanced the penetration of solvents and lipid extraction.

中文翻译：

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脉冲电场预处理在提高废水中蛋白核小球藻脂质提取中的应用

脂质提取是微藻生产生物柴油的关键步骤，然而，由于细胞破碎困难和溶剂毒性，传统提取方法的应用受到限制。在这项工作中，使用脉冲电场 (PEF) 的预处理方法主要应用于废水中生长的微藻蛋白核糖体小球藻的脂质提取过程。PEF预处理后，蛋白核糖体脂肪酸甲酯的产率比传统超声波预处理高12.0%。结果表明PEF是一种有效的细胞破碎方法。荧光染色和扫描电镜结果表明，脉冲电场作用下微藻细胞膜的完整性受到破坏，增强了溶剂的渗透和脂质提取。