We have developed a hybrid nanomaterial based on both of carbon quantum dots(CQDs)/gold nanoparticles(AuNPs), such that the cheapness and versatility on the CQDs is directly combined with the inertness and electrochemical activity of the AuNPs to create a new electrochemical biosensor. Here, for the first time, we demonstrate how this interesting materials combination can be applied in the development of an enzymatic biosensor that is easily manufacturable using standard semiconductor processing methods. To demonstrate the potential and performance of the CQDs as an immobilization matrix, glucose oxidase(GOx) enzyme was chosen as a model system. The analytical performance of the developed biosensor was examined using chronoamperometry and the developed CQDs/AuNPs-GOx biosensor exhibited a sensitivity of 47.24 μA mM⁻¹ cm⁻² and a detection limit of 17 μM (S/N = 3) with a linear response to glucose ranging from 0.05 mM to 2.85 mM (R = 0.987). Furthermore, this new biosensor showed a high level of reproducibility and was also shown to be very selective towards glucose in the presence of the possible interference species ascorbic acid, uric acid and acetaminophen. Finally, it is demonstrated that the sensor is also capable of reliably detecting glucose levels in human serum.

我们已经开发了一种基于碳量子点（CQDs）/金纳米颗粒（AuNPs）的杂化纳米材料，从而将CQDs的便宜性和多功能性与AuNPs的惰性和电化学活性直接结合在一起，从而创建了一个新的电化学生物传感器。在这里，我们首次展示了如何将这种有趣的材料组合应用于酶促生物传感器的开发，该传感器可以使用标准的半导体加工方法轻松制造。为了证明CQD作为固定基质的潜力和性能，选择了葡萄糖氧化酶（GOx）酶作为模型系统。使用计时电流法检查了开发的生物传感器的分析性能，开发的CQDs / AuNPs-GOx生物传感器显示出47.24μAmM ^-1的灵敏度cm ^-2且检测限为17μM（S / N = 3），对葡萄糖的线性响应范围为0.05 mM至2.85 mM（R = 0.987）。此外，这种新型生物传感器显示出高水平的可重复性，并且在存在可能的干扰物质抗坏血酸，尿酸和对乙酰氨基酚的情况下，对葡萄糖的选择性也很高。最后，证明该传感器还能够可靠地检测人血清中的葡萄糖水平。