Although several new types of synthetic cell-like entities are now available, their structural integration into spatially interlinked prototissues that communicate and display coordinated functions remains a considerable challenge. Here we describe the programmed assembly of synthetic prototissue constructs based on the bio-orthogonal adhesion of a spatially confined binary community of protein–polymer protocells, termed proteinosomes. The thermoresponsive properties of the interlinked proteinosomes are used collectively to generate prototissue spheroids capable of reversible contractions that can be enzymatically modulated and exploited for mechanochemical transduction. Overall, our methodology opens up a route to the fabrication of artificial tissue-like materials capable of collective behaviours, and addresses important emerging challenges in bottom-up synthetic biology and bioinspired engineering.

尽管现在可以使用几种新型的合成细胞样实体，但是它们在结构上整合到空间上相互联系的原型组织中，以沟通和展示协调的功能仍然是一个巨大的挑战。在这里，我们根据蛋白质聚合物原细胞的空间受限的二元群落的生物正交粘附力，描述了合成的原组织构建体的程序化组装，该蛋白质群落称为蛋白体。相互连接的蛋白质体的热响应特性共同用于生成能够逆向收缩的原组织球体，该原球体可以酶促调节并用于机械化学转导。总体而言，我们的方法为制造具有集体行为的人造组织样材料开辟了一条道路，