Legumes establish symbiotic relationships with different microorganisms, which could function as plant growth promotion microorganisms (PGPM). The finding of new PGPM strains is important to increase plant production avoiding or diminishing the use of industrial fertilizers. Thus, in this work we evaluated the plant growth promotion traits of ten strains isolated from Mimosa pudica root nodules. According to the 16S rDNA sequence, the microorganisms were identified as Enterobacter sp. and Serratia sp. To the best of our knowledge this is the first report describing and endophytic interaction between Mimosa pudica and Enterobacter sp. These strains have some plant growth promoting traits such as phosphate solubilization, auxin production and cellulase and chitinase activity. Strains identified as Serratia sp. inhibited the growth of the phytopathogenic fungi Fusarium sp., and Alternaria solani and the oomycete Phytophthora capsici. According to their biochemical characteristics, three strains were selected to test their plant growth promoting activity in a medium with an insoluble phosphate source. These bacteria show low specificity for their hosts as endophytes, since they were able to colonize two very different legumes: Phaseolus vulgaris and M. pudica. Seedlings of P. vulgaris were inoculated and grown for fifteen days. Enterobacter sp. NOD1 and NOD10, promoted growth as reflected by an increase in shoot height as well as an increase in the size and emergence of the first two trifolia. We could localize NOD5 as an endophyte in roots in P. vulgaris by transforming the strain with a Green Fluorescent Protein carrying plasmid. Experiments of co-inoculation with different Rhizobium etli strains allowed us to discard that NOD5 can fix nitrogen in the nodules formed by a R. etli Fix⁻ strain. The isolates described in this work show biotechnological potential for plant growth promoting activity and production of indoleacetic acid and siderophores.

豆科植物与不同的微生物建立共生关系，可以作为植物生长促进微生物（PGPM）。寻找新的PGPM菌株对于避免或减少工业肥料的使用对提高植物产量非常重要。因此，在这项工作中，我们评估了从含羞草根瘤中分离的十个菌株的植物生长促进性状。根据16S rDNA序列，将微生物鉴定为肠杆菌属。和沙雷氏菌 据我们所知，这是第一份描述含羞草和肠杆菌之间的内生相互作用的报告。sp。这些菌株具有一些促进植物生长的特性，例如磷酸盐增溶，植物生长素的产生以及纤维素酶和几丁质酶的活性。鉴定为沙雷氏菌的菌株。抑制了植物病原真菌镰刀菌和链格孢菌和卵菌疫霉的生长。根据其生化特性，选择了三种菌株以在具有不溶性磷酸盐源的培养基中测试其促进植物生长的活性。这些细菌对它们作为内生菌的宿主显示出较低的特异性，因为它们能够在两种截然不同的豆科植物中定居：菜豆和布丁芽孢杆菌。普通百日草的幼苗接种并生长十五天。肠杆菌属。NOD1和NOD10促进了生长，这反映在枝条高度的增加以及前两个三叶草的大小和出苗的增加上。通过用携带绿色荧光蛋白的质粒转化菌株，我们可以将NOD5定位为寻常型假单胞菌根中的内生菌。共接种不同的实验根瘤菌etli菌株允许我们丢弃NOD5可以在由所形成的结节固氮R. etli修复^-应变。在这项工作中描述的分离株显示出促进植物生长活性和吲哚乙酸和铁载体生产的生物技术潜力。