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Hybrid CuCoO–GO enables ultrasensitive detection of antibiotics with enhanced laser desorption/ionization at nano-interfaces†
Chemical Science ( IF 7.6 ) Pub Date : 2018-10-08 00:00:00 , DOI: 10.1039/c8sc03692f Enhui Wu 1 , Kun Feng 1 , Rui Shi 1 , Rui Lv 1 , Fuzhong Ouyang 1 , Shawn S C Li 2 , Jun Zhong 1 , Jian Liu 1
Chemical Science ( IF 7.6 ) Pub Date : 2018-10-08 00:00:00 , DOI: 10.1039/c8sc03692f Enhui Wu 1 , Kun Feng 1 , Rui Shi 1 , Rui Lv 1 , Fuzhong Ouyang 1 , Shawn S C Li 2 , Jun Zhong 1 , Jian Liu 1
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The soaring concerns globally on antibiotic overuse have made calls for the development of rapid and sensitive detection methods urgent. Here we report that the hybrid CuCoO–GO matrix allows for sensitive detection of various antibiotics in combination with MALDI TOF MS. The new matrix is composed of few-layered GO nanosheets decorated with CuCoO nanoparticles with an average size of 10 nm, and exhibits excellent aqueous suspensibility. Accurate quantitation of the sulfonamide antibiotics in milk samples have been demonstrated using a CuCoO–GO matrix and a stable isotope (C13)-labeled analyte as the internal standard. Our experiments have achieved lower limits of detection (LOD) by several hundred fold for the detection of a panel of representative antibiotics, in comparison with the literature reports. Both intrabacterial and extrabacterial residual antibiotics can be sensitively detected with our method. We have further investigated the molecular mechanism of the enhanced desorption/ionization efficiency by the CuCoO–GO matrix with synchrotron radiation techniques for the first time. This work provides a sensitive matrix enabling MALDI-TOF MS to be applied in small molecular analysis, but also presents a distinct perspective on the mechanism behind the material functions.
中文翻译：
混合 CuCoO-GO 能够在纳米界面处通过增强的激光解吸/电离来超灵敏地检测抗生素†
全球对抗生素过度使用的担忧日益高涨，迫切需要开发快速和灵敏的检测方法。在这里，我们报告了混合 CuCoO-GO 基质结合 MALDI TOF MS 可以灵敏检测各种抗生素。新基质由平均尺寸为 10 nm 的 CuCoO 纳米颗粒装饰的少层 GO 纳米片组成，具有优异的水悬浮性。使用 CuCoO-GO 基质和稳定同位素（C 13)-标记的分析物作为内标。与文献报道相比，我们的实验已将一组代表性抗生素的检测限 (LOD) 降低了数百倍。我们的方法可以灵敏地检测出细菌内和细菌外残留的抗生素。我们首次利用同步辐射技术进一步研究了CuCoO-GO基质提高解吸/电离效率的分子机制。这项工作提供了一种敏感的基质，使 MALDI-TOF MS 能够应用于小分子分析，同时也对材料功能背后的机制提出了独特的观点。
更新日期：2018-10-08
中文翻译：
混合 CuCoO-GO 能够在纳米界面处通过增强的激光解吸/电离来超灵敏地检测抗生素†
全球对抗生素过度使用的担忧日益高涨，迫切需要开发快速和灵敏的检测方法。在这里，我们报告了混合 CuCoO-GO 基质结合 MALDI TOF MS 可以灵敏检测各种抗生素。新基质由平均尺寸为 10 nm 的 CuCoO 纳米颗粒装饰的少层 GO 纳米片组成，具有优异的水悬浮性。使用 CuCoO-GO 基质和稳定同位素（C 13)-标记的分析物作为内标。与文献报道相比，我们的实验已将一组代表性抗生素的检测限 (LOD) 降低了数百倍。我们的方法可以灵敏地检测出细菌内和细菌外残留的抗生素。我们首次利用同步辐射技术进一步研究了CuCoO-GO基质提高解吸/电离效率的分子机制。这项工作提供了一种敏感的基质，使 MALDI-TOF MS 能够应用于小分子分析，同时也对材料功能背后的机制提出了独特的观点。
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