An AceDAN–porphyrin(Zn) dyad for fluorescence imaging and photodynamic therapy via two-photon excited FRET†,Inorganic Chemistry Frontiers

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An AceDAN–porphyrin(Zn) dyad for fluorescence imaging and photodynamic therapy via two-photon excited FRET†
Inorganic Chemistry Frontiers ( IF 6.1 ) Pub Date : 2018-10-06 00:00:00 , DOI: 10.1039/c8qi00705e
Mengliang Zhu _{1,

2,

3,

4,

5} , Chaorui Su _{1,

2,

3,

4,

5} , Peipei Xing _{1,

2,

3,

4,

5} , Yabin Zhou _{3,

4,

5,

6} , Lei Gong _{1,

2,

3,

4,

5} , Jinghui Zhang _{1,

2,

3,

4,

5} , Hongwu Du _{3,

4,

5,

6} , Yongzhong Bian _{1,

2,

3,

4,

5} , Jianzhuang Jiang _{1,

2,

3,

4,

5}

Affiliation

The recent development of the two-photon technique offers a unique opportunity to improve the depth of imaging and treatment in photodynamic therapy (PDT). Herein, we designed a fluorescence resonance energy transfer (FRET) based AceDAN–porphyrin(Zn) dyad for two-photon excited fluorescence imaging and PDT of cancer cells simultaneously, in which the AceDAN moiety was selected as the two-photon absorption donor and the porphyrin(Zn) moiety served as the energy acceptor. Upon one-photon or two-photon excitation, the excited state energy of the AceDAN donor was transferred to the porphyrin(Zn) acceptor with high efficiency (η_EET = 98%), where red fluorescence and singlet oxygen were generated. Dyad 1 exhibited high photocytotoxicity towards A549 cells (IC₅₀ = 4.3 μM) with rapid cellular uptake while displaying low dark-cytotoxicity. Furthermore, by combining the advantages of two-photon excitation with a porphyrin(Zn) photosensitizer, the AceDAN–porphyrin(Zn) dyad has been successfully applied to A549 cells for imaging and photodynamic therapy under two-photon laser irradiation.

中文翻译：

通过双光子激发的FRET 进行荧光成像和光动力疗法的AceDAN–卟啉（Zn）双标记†

两光子技术的最新发展为改善光动力疗法（PDT）的成像和治疗深度提供了独特的机会。在本文中，我们设计了基于荧光共振能量转移（FRET）的AceDAN-卟啉（Zn）二聚体，用于同时进行癌细胞的双光子激发荧光成像和PDT，其中AceDAN部分被选作双光子吸收供体，卟啉（Zn）部分用作能量受体。在单光子或双光子激发时，供体AceDAN的激发态能量转移到具有高效率的卟啉（Zn）的受体（η _EET = 98％），其中，产生红色荧光和单线态氧。Dyad 1对A549细胞表现出高光细胞毒性（IC ₅₀= 4.3μM），细胞吸收迅速，而暗细胞毒性低。此外，通过结合双光子激发与卟啉（Zn）光敏剂的优点，AceDAN–卟啉（Zn）二聚体已成功应用于A549细胞，以在双光子激光照射下进行成像和光动力疗法。

更新日期：2018-10-06

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