Two up-flow anaerobic sludge blanket (UASB) reactors (RM with 10 g Fe L⁻¹ magnetite, RB without magnetite) feeding with synthetic wastewater were built to investigate the effect of magnetite addition on anaerobic digestion (AD) performance during start-up and starvation recovery stages. With the magnetite addition, the COD removal efficiency and biogas production during the two stages were enhanced, and the recovery time of RM was shortened by about 50%. The reduced synthesis of riboflavin and heme c along with enhanced sludge conductivity of RM indicated that magnetite could replace their roles for efficient extracellular electron transfer (EET), which favors the growth of anaerobes. Microbial community analysis showed that potential syntrophic partners like Syntrophaceae and Methanothrix were enriched in RM during the recovery stage, and the performance was improved with quick responsiveness. Results demonstrated that addition of conductive materials like magnetite could improve the stability and restorability of AD process efficiently.

建造了两个合成废水供料的上流厌氧污泥层反应器（UASB）反应器（RM含10 g Fe L ^-1磁铁矿，RB不带磁铁矿），以研究磁铁矿添加对启动过程中厌氧消化（AD）性能的影响和饥饿恢复阶段。加入磁铁矿后，两个阶段的COD去除效率和沼气产量均得到提高，RM的回收时间缩短了约50％。核黄素和血红素c的合成减少，以及RM的污泥电导率提高，表明磁铁矿可以代替它们在有效的细胞外电子转移（EET）中的作用，从而有利于厌氧菌的生长。微生物群落分析表明这样的潜在合作伙伴互养Syntrophaceae和甲氨蝶呤在恢复阶段富含RM，通过快速响应提高了性能。结果表明，添加磁铁矿等导电材料可以有效提高AD工艺的稳定性和可恢复性。