Nitrogen removal and microbial interactions in two combined nitritation and Anammox systems with or without the addition of organics were examined. Two systems were successfully started up by adopting intermittent aeration. Organics addition deteriorated nitrogen removal, and total inorganic nitrogen and ammonium removal percentages decreased by 16.4% and 26.3%, respectively. Organics addition promoted the growth of Chloroflexi and Proteobacteria while suppressed the growth of Candidatus Kuenenia. Organics addition decreased activities of fatty acid biosynthesis and metabolism, amino acid metabolism and biofilm formation, while increased activities of steroid metabolism and glycosaminoglycan biosynthesis. Heterotrophs and Candidatus Kuenenia might interact with other organisms by using diverse quorum sensing systems. Chloroflexi and Proteobacteria interacted with Candidatus Kuenenia in nitrogen metabolism and biofilm formation. Proteobacteria played a key role in building a nitrite loop with Candidatus Kuenenia and nitrifiers. These results clarified microbial interactions in the autotrophic nitrogen removal process and advance its application.

在两个有机化和厌氧氨氮混合系统中，在添加或不添加有机物的情况下，研究了氮的去除和微生物之间的相互作用。通过采用间歇曝气成功地启动了两个系统。有机物的添加降低了氮的去除，总无机氮和铵的去除百分比分别降低了16.4％和26.3％。有机物添加促进生长绿弯菌门和变形菌门，同时抑制的生长暂定Kuenenia。有机物的添加减少了脂肪酸生物合成和代谢，氨基酸代谢和生物膜形成的活性，而增加了类固醇代谢和糖胺聚糖生物合成的活性。异养菌和念珠菌Kuenenia可能通过使用不同的群体感应系统与其他生物相互作用。绿弯菌门和变形菌相互作用与暂定氮代谢和生物膜形成Kuenenia。变形菌在建设有亚硝酸盐循环发挥了关键作用暂定Kuenenia和硝化细菌。这些结果阐明了微生物在自养氮去除过程中的相互作用，并促进了其应用。