Commercial fuel ethanol fermentations suffer from microbial contaminants, particularly species of Lactobacillus that may persist as antibiotic-resistant biofilms. In this study, culture supernatants from 54 strains of Bacillus known to produce lipopeptides were tested for inhibition of biofilm formation by Lactobacillus fermentum, L. plantarum, and L. brevis strains previously isolated as biofilm-forming contaminants of a commercial fuel ethanol facility. Eleven Bacillus strains inhibited biofilm formation by all three strains by at least 65% of controls. None of these strains inhibited Saccharomyces cerevisiae. Three strains also significantly inhibited planktonic cultures of Lactobacillus. Culture supernatants from B. nakamurai strain NRRL B-41091 were particularly effective. Inhibition was bacteriostatic rather than bacteriocidal, and appeared to be specific for strains of Lactobacillus. Furthermore, the inhibitor from B. nakamurai was shown to prevent stuck fermentations in a corn mash model fermentation system of S. cerevisiae contaminated with L. fermentum.

商业燃料乙醇发酵会受到微生物污染物的影响，尤其是乳杆菌属物种，它们可能会以抗药性生物膜的形式持续存在。在这项研究中，测试了54株已知会产生脂肽的芽孢杆菌的培养上清液对发酵乳杆菌，植物乳杆菌和短乳杆菌的生物膜形成的抑制作用，这些菌株以前是作为商业燃料乙醇设施的生物膜形成污染物而分离的。至少有65％的对照抑制了11种芽孢杆菌菌株对所有这三种菌株的生物膜形成的抑制作用。这些菌株均不能抑制啤酒酵母。三种菌株也显着抑制乳酸菌的浮游培养。来自中村芽孢杆菌NRRL B-41091菌株的培养上清液特别有效。抑制作用是抑菌的而不是杀菌的，并且似乎对乳酸杆菌菌株具有特异性。此外，来自抑制剂B. nakamurai显示出防止卡住发酵中的玉米醪发酵模型系统酿酒酵母污染发酵乳杆菌。