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Development and validation of a ready to use cryo-EROD assay for the standardized screening of dioxins and dioxin-like compounds in foodstuffs
Food and Chemical Toxicology ( IF 3.9 ) Pub Date : 2018-10-06 , DOI: 10.1016/j.fct.2018.10.023
Walkiria Levy , Karl-Werner Schramm , Florian Mertes , Bernhard Henkelmann , Martina Maywald , Peter Uciechowski , Alexander Loa , Johannes Haedrich , Ines Thiem , Henner Hollert , Roland Goerlich , Thorsten Bernsmann , Lothar Rink

Recent European regulations have indicated the need for new bioanalytical screening methods capable of monitoring dioxin and dioxin-like compounds in foodstuffs and environmental samples, cost-effectively and with a quicker turnaround. Cryo-cells of the hepatic H4IIE line preserved in 96-well plates were exposed to sample extracts prepared from various foodstuffs and analysed for their content of dioxins and dioxin-like compounds by means of the 7-Ethoxyresorufin-O-Deethylase (EROD)-assay in two laboratories. Assay data were compared between both laboratories and results from instrumental analysis used as a confirmatory method. Additionally, cut-off values for the different studied matrices were derived. The current European regulation regarding methods of analysis for the control of foodstuffs was applied with the aim of determining the feasibility of the cryo-methodology. Results obtained in both laboratories were in congruence with the required validation parameters of the Commission Regulation (EU) No 2017/644. Cut-off values should be established matrix-dependent to reduce the rate of false compliant results and to keep the rate of false non-compliant results under control. In summary, the ready-to-use cryo-assay method for the bioanalytical screening of foodstuffs in control laboratories without cell-culture facilities has successfully proven to be accurate, far quicker and more cost effective than current methods.



中文翻译:

用于标准食品中二恶英和二恶英类化合物标准筛分的即用型EROD检测方法的开发和验证

最近的欧洲法规已经表明需要新的生物分析筛选方法,该方法能够以经济高效且更快速的方式监测食品和环境样品中的二恶英和类二恶英化合物。将保存在96孔板中的H4IIE肝细胞冷冻细胞暴露于从各种食品中制备的样品提取物中,并通过7-乙氧基间苯二酚O分析其二恶英和二恶英样化合物的含量。-在两个实验室中进行脱乙基酶(EROD)分析。在两个实验室之间对测定数据进行了比较,并将仪器分析的结果用作验证方法。此外,得出了不同研究矩阵的临界值。为了确定冷冻方法的可行性,应用了当前有关食品控制分析方法的欧洲法规。在两个实验室中获得的结果均与欧盟法规(EU)第2017/644号所要求的验证参数一致。应建立与矩阵有关的临界值,以降低错误合规结果的比率,并使错误不合规结果的比率得到控制。总之,

更新日期:2018-10-06
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