AbstractGlutathione-capped water-soluble CuInS/ZnS quantum dots (QDs) were prepared by a microwave-assisted method. Their fluorescence, with excitation/emission peaks at 380/570 nm, is found to be quenched by hydrogen peroxide (H2O2) that is produced by the uricase catalyzed oxidation of uric acid (UA) and oxygen. The findings are used in a quenchometric method for the determination of UA. The effects of different ligands on the QDs, of pH value, buffers, enzyme ratio and reaction time were optimized. The detection limit for UA is 50 nM which is lower than other QD-based method, and the detection ranges extends from 0.25–4.0 μM. The assay is simple and sensitive, and no further modification of the QDs is required. Graphical abstractᅟ

中文翻译：

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使用水溶性 CuInS/ZnS 量子点作为荧光探针酶法测定尿酸

摘要 采用微波辅助方法制备了谷胱甘肽封端的水溶性 CuInS/ZnS 量子点 (QDs)。发现它们的荧光在 380/570 nm 处具有激发/发射峰，可被过氧化氢 (H2O2) 淬灭，过氧​​化氢 (H2O2) 是由尿酸酶催化的尿酸 (UA) 和氧气氧化产生的。研究结果用于测定 UA 的淬灭法。优化了不同配体对QDs、pH值、缓冲液、酶比和反应时间的影响。UA 的检测限为 50 nM，低于其他基于 QD 的方法，检测范围为 0.25–4.0 μM。该测定简单且灵敏，无需进一步修改量子点。图形摘要ᅟ