The biosynthesis of two polyketides, atranorin and fumarprotocetraric acid, produced from a lichen-forming fungus, Cladonia rangiferina (L.) F. H. Wigg. was correlated with the expression of eight fungal genes (CrPKS1, CrPKS3, CrPKS16, Catalase (CAT), Sugar Transporter (MFsug), Dioxygenase (YQE1), C2H2 Transcription factor (C2H2), Transcription Factor PacC (PacC), which are thought to be involved in polyketide biosynthesis, and one algal gene, NAD-dependent deacetylase sirtuin 2 (AsNAD)), using laser microdissection (LMD). The differential gene expression levels within the thallus tissue layers demonstrate that the most active region for potential polyketide biosynthesis within the lichen is the outer apical region proximal to the photobiont but some expression also occurs in reproductive tissue. This is the first study using laser microdissection to explore gene expression of these nine genes and their location of expression; it provides a proof-of-concept for future experiments exploring tissue-specific gene expression within lichens; and it highlights the utility of LMD for use in lichen systems.

中文翻译：

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聚酮合酶和其他相关基因在地衣中的组织特异性定位，Cladonia rangiferina，使用激光显微切割

由形成地衣的真菌 Cladonia rangiferina (L.) FH Wigg 产生的两种聚酮化合物 atranorin 和延胡索原四酸的生物合成。与八种真菌基因（CrPKS1、CrPKS3、CrPKS16、过氧化氢酶（CAT）、糖转运蛋白（MFsug）、双加氧酶（YQE1）、C2H2 转录因子（C2H2）、转录因子 PacC（PacC）的表达相关）使用激光显微切割 (LMD)，参与聚酮化合物的生物合成，以及一种藻类基因，NAD 依赖性脱乙酰酶 Sirtuin 2 (AsNAD))。叶状体组织层内的差异基因表达水平表明，地衣内潜在聚酮化合物生物合成最活跃的区域是靠近光生物的外顶端区域，但在生殖组织中也会发生一些表达。这是第一次使用激光显微切割来探索这九个基因的基因表达及其表达位置的研究；它为未来探索地衣内组织特异性基因表达的实验提供了概念验证；它强调了 LMD 在地衣系统中的应用。