Chemically defined cell culture media is a complex mixture of molecules used for cellular metabolism and growth. Decisions surrounding which molecules to include in a formulation and their optimal concentration is a major endeavor in biomanufacturing. Cellular metabolic complexity and stringent quality requirements that vary across cell lines require time-consuming, iterative experimentation to optimize media formulation. Exacerbating this is the orders-of-magnitude concentration differences among media components making media characterization difficult. This review describes the challenges, advances and strategies currently used to optimize media formulations as well as future opportunities for improvement.

化学定义的细胞培养基是用于细胞代谢和生长的分子的复杂混合物。围绕制剂中包括哪些分子及其最佳浓度的决定是生物制造的主要努力。细胞代谢复杂性和严格的质量要求因细胞系而异，需要耗时的迭代实验来优化培养基配方。加剧的是媒体成分之间的数量级浓度差异，使媒体表征变得困难。这篇综述描述了目前用于优化媒体配方的挑战，进展和策略，以及未来的改进机会。