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Selective electrohydrodynamic concentration of waterborne parasites on a chip†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-10-04 00:00:00 , DOI: 10.1039/c8lc00840j Romuald Lejard-Malki 1, 2, 3, 4, 5 , Jérôme Follet 1, 2, 3, 4, 5 , Alexis Vlandas 1, 2, 3, 4, 5 , Vincent Senez 1, 2, 3, 4, 5
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-10-04 00:00:00 , DOI: 10.1039/c8lc00840j Romuald Lejard-Malki 1, 2, 3, 4, 5 , Jérôme Follet 1, 2, 3, 4, 5 , Alexis Vlandas 1, 2, 3, 4, 5 , Vincent Senez 1, 2, 3, 4, 5
Affiliation
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Concentrating diluted samples is a key step to improve detection capabilities. The wise use of scaling laws shows the advantages of working with sub-microliter-sized samples. Rapid progress in MEMS technologies has driven the design of integrated platforms performing many biochemical operations. Here we report a new concentrator device based on electro-hydrodynamic forces which can be easily integrated into electrowetting-on-dielectric (EWOD) platforms. This approach is label-free and applicable to a wide range of micro-objects. The detection and analysis of two common waterborne parasites, Cryptosporidium and Giardia, is a perfect test case due to their global health relevance. By fully controlling the interplay of the various forces acting on the micron-sized Cryptosporidium parvum and Cryptosporidium muris oocysts, we show that it is possible to concentrate them on the side of a 10 μL initial drop and then extract them efficiently from a droplet of a few hundred nanoliters. We performed a finite element modeling of the forces acting on the parasites' oocysts to optimize the electrodes' shapes. We obtained state-of-the-art concentration factors of 12 ± 0.4 times and 2 to 4 times in the sub-region of the drop and the extracted droplet, respectively, with an efficiency of 70 ± 6%. Furthermore, this device had the ability to selectively concentrate parasites of different species out of a mix. We demonstrated this by segregating C. parvum oocysts from either Giardia lamblia cysts or its related species, C. muris oocysts.
中文翻译：
芯片上水性寄生虫的选择性电流体动力学浓度†
浓缩稀释的样品是提高检测能力的关键步骤。缩放定律的明智使用显示了处理亚微升大小样品的优势。MEMS技术的飞速发展推动了执行许多生化操作的集成平台的设计。在这里，我们报告了一种基于电液动力的新型集中器设备，该设备可轻松集成到电介质上电润湿（EWOD）平台中。这种方法是无标签的，适用于广泛的微对象。由于对全球健康的重要性，对两种常见的水生寄生虫（隐孢子虫和贾第虫）的检测和分析是一个完美的测试案例。通过完全控制作用在微米尺寸上的各种力的相互作用隐孢子虫和鼠隐孢子虫卵囊，我们证明有可能将它们浓缩在10μL初始液滴的一侧，然后从几百纳升的液滴中有效地提取它们。我们对作用在寄生虫卵囊上的力进行了有限元建模，以优化电极的形状。我们在液滴的子区域和提取的液滴的子区域中分别获得了12±0.4倍和2至4倍的最新浓度因子，效率为70±6％。此外，该装置具有从混合物中选择性地浓缩不同物种的寄生虫的能力。我们通过从贾第鞭毛虫孢囊或其相关物种中分离出小球藻卵囊来证明这一点，C.鼠类卵囊。
更新日期：2018-10-04
中文翻译：
芯片上水性寄生虫的选择性电流体动力学浓度†
浓缩稀释的样品是提高检测能力的关键步骤。缩放定律的明智使用显示了处理亚微升大小样品的优势。MEMS技术的飞速发展推动了执行许多生化操作的集成平台的设计。在这里，我们报告了一种基于电液动力的新型集中器设备，该设备可轻松集成到电介质上电润湿（EWOD）平台中。这种方法是无标签的，适用于广泛的微对象。由于对全球健康的重要性，对两种常见的水生寄生虫（隐孢子虫和贾第虫）的检测和分析是一个完美的测试案例。通过完全控制作用在微米尺寸上的各种力的相互作用隐孢子虫和鼠隐孢子虫卵囊，我们证明有可能将它们浓缩在10μL初始液滴的一侧，然后从几百纳升的液滴中有效地提取它们。我们对作用在寄生虫卵囊上的力进行了有限元建模，以优化电极的形状。我们在液滴的子区域和提取的液滴的子区域中分别获得了12±0.4倍和2至4倍的最新浓度因子，效率为70±6％。此外，该装置具有从混合物中选择性地浓缩不同物种的寄生虫的能力。我们通过从贾第鞭毛虫孢囊或其相关物种中分离出小球藻卵囊来证明这一点，C.鼠类卵囊。
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