Bichromophoric Sensors for Ratiometric Measurements of Molecular Microenvironments through the Interplay of Charge Transfer and Energy Transfer Channels.,ChemPlusChem

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Bichromophoric Sensors for Ratiometric Measurements of Molecular Microenvironments through the Interplay of Charge Transfer and Energy Transfer Channels.
ChemPlusChem ( IF 3.0 ) Pub Date : 2018-10-29 , DOI: 10.1002/cplu.201800333
Andres Arroyo-Pieck ₁ , Daniela Araiza-Olivera ₂ , Jorge Peon ₁

Affiliation

A new molecular architecture was designed to amplify the sensitivity of bichromophoric probes, in which two sequential kinetic competitions of photophysical channels were used to define the emission yield of the lower energy chromophore. Additionally, the emission from both chromophores can be used for ratiometric measurements, which are concentration independent. Two sensors were synthesized to demonstrate the concept, coupling a boron-dipyrromethene (BODIPY) dye and a cyanine dye. Both the energy transfer from the BODIPY to the cyanine and the cyanine radiative channel compete with a charge transfer state formation, giving the cyanine emission intensity a twofold dependence on polarity. This was confirmed with steady state and time-resolved spectroscopies. Also, the large spectral gap between the two emissions (approx. 280 nm) makes the ratiometric measurements crosstalk-free. The use of the sensors in live cells was demonstrated through the staining and imaging of SK-LU-1 lung cells under normal and apoptotic conditions.

中文翻译：

通过电荷转移通道和能量转移通道之间的相互作用进行分子微环境比例测量的双色传感器。

设计了一种新的分子结构来放大双色探针的灵敏度，其中使用了两个连续的光物理通道动力学竞争来确定较低能发色团的发射量。另外，两个生色团的发射都可用于比率测量，而比率测量与浓度无关。合成了两种传感器来证明这一概念，即偶联了一种硼二吡咯亚甲基（BODIPY）染料和一种花菁染料。从BODIPY到花青的能量转移和花青的辐射通道都与电荷转移状态的形成竞争，从而使花青的发射强度对极性的依赖性增加了两倍。稳定状态和时间分辨光谱学证实了这一点。同样，两种发射之间的光谱差距较大（约 280 nm）使比率测量无串扰。通过在正常和凋亡条件下对SK-LU-1肺细胞进行染色和成像，证明了传感器在活细胞中的使用。

更新日期：2018-10-29

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