Chromosome 17 Missing Proteins: Recent Progress and Future Directions as Part of the neXt-MP50 Challenge,Journal of Proteome Research

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Chromosome 17 Missing Proteins: Recent Progress and Future Directions as Part of the neXt-MP50 Challenge
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-10-23 , DOI: 10.1021/acs.jproteome.8b00442
Omer Siddiqui _{1,

2} , Hongjiu Zhang ₂ , Yuanfang Guan _{2,

3} , Gilbert S. Omenn _{2,

3,

4}

Affiliation

The Chromosome-centric Human Proteome Project (C-HPP), announced in September 2016, is an initiative to accelerate progress on the detection and characterization of neXtProt PE2,3,4 “missing proteins” (MPs) with a mandate to each chromosome team to find about 50 MPs over 2 years. Here we report major progress toward the neXt-MP50 challenge with 43 newly validated Chr 17 PE1 proteins, of which 25 were based on mass spectrometry, 12 on protein–protein interactions, 3 on a combination of MS and PPI, and 3 with other types of data. Notable among these new PE1 proteins were five keratin-associated proteins, a single olfactory receptor, and five additional membrane-embedded proteins. We evaluate the prospects of finding the remaining 105 MPs coded for on Chr 17, focusing on mass spectrometry and protein–protein interaction approaches. We present a list of 35 prioritized MPs with specific approaches that may be used in further MS and PPI experimental studies. Additionally, we demonstrate how in silico studies can be used to capture individual peptides from major data repositories, documenting one MP that appears to be a strong candidate for PE1. We are close to our goal of finding 50 MPs for Chr 17.

中文翻译：

17号染色体缺失蛋白：neXt-MP50挑战的一部分，最新进展和未来方向

2016年9月宣布了以染色体为中心的人类蛋白质组计划（C-HPP），该计划旨在加快neXtProt PE2、3、4“缺失蛋白”（MPs）的检测和表征进展，并赋予每个染色体小组以授权在2年内找到大约50名议员。在这里，我们报告了neXt-MP50挑战的主要进展，其中包括43种新验证的Chr 17 PE1蛋白，其中25种基于质谱，12种基于蛋白质-蛋白质相互作用，3种基于MS和PPI组合，3种与其他类型数据的。在这些新的PE1蛋白中，值得注意的是5种角蛋白相关蛋白，单个嗅觉受体和5种其他膜包埋蛋白。我们评估寻找剩余的105 Chr编码的Chr 17的前景，重点是质谱法和蛋白质-蛋白质相互作用方法。我们列出了35种具有特定方法的优先MP，这些方法可以用于进一步的MS和PPI实验研究。此外，我们演示了如何利用计算机模拟研究从主要数据存储库中捕获单个肽，并证明了一种MP似乎是PE1的强力候选者。我们已接近为Chr 17找到50 MP的目标。

更新日期：2018-10-23

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