A ternary deep eutectic solvent (TDES) was used as both template and functional monomer in the synthesis of TDES-based molecularly imprinted polymers (TDES-MIPs). A meticulous miniaturized solid-phase microextraction (mini-SPME) method followed by high-performance liquid chromatography (HPLC) were used for the optimal speciation of 3,4-dihydroxybenzoic acid (3,4-DHBA) in the needle of a syringe system with response surface methodology (RSM). Under the optimal conditions for the determination of 3,4-DHBA (amount of adsorbent (2 mg), sample volume (1 mL), cycles for adsorption and desorption (6)), the actual extraction amount was 8.46 μg g⁻¹. The limits of detection (LODs, S/N = 3) for 3,4-DHBA in Ilex chinensis Sims were 0.26–0.31 μg mL⁻¹, and the intra-day and inter-day precision (relative standard deviations, n = 4) after spiking with 5 μg mL⁻¹, 100 μg mL⁻¹, and 200 μg mL⁻¹ were both less than 4.21%. The meticulous method (TDES-MIP-mini-SPME) combined with RSM offers a significant advance over existing methods, because of the meticulous operation and excellent selectivity of 3,4-DHBA from complex samples.

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在基于TDES的分子印迹聚合物（TDES-MIP）的合成中，将三元深共熔溶剂（TDES）用作模板和功能单体。细微的微型固相微萃取（mini-SPME）方法，然后用高效液相色谱（HPLC）用于注射器系统针头中3,4-二羟基苯甲酸（3,4-DHBA）的最佳形态使用响应面方法（RSM）。在确定3,4-DHBA的最佳条件下（吸附剂量（2 mg），样品量（1 mL），吸附和解吸循环（6）），实际提取量为8.46μgg ^-1。冬青树Sims中3,4-DHBA的检出限（LODs，S / N = 3）为0.26-0.31μgmL ^-1和日内和日间精密度（相对标准偏差，Ñ 用5μg毫升尖峰之后= 4）^-1，100微克毫升^-1，和200μg毫升^-1均小于4.21％。细致的方法（TDES-MIP-mini-SPME）与RSM相结合，比现有方法有了重大进步，这是因为细致的操作和对复杂样品中3,4-DHBA的优异选择性。

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