当前位置: X-MOL 学术Anal. Bioanal. Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Selective extraction of 3,4-dihydroxybenzoic acid in Ilex chinensis Sims by meticulous mini-solid-phase microextraction using ternary deep eutectic solvent-based molecularly imprinted polymers
Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2018-10-03 , DOI: 10.1007/s00216-018-1406-y
Guizhen Li , Kyung Ho Row

A ternary deep eutectic solvent (TDES) was used as both template and functional monomer in the synthesis of TDES-based molecularly imprinted polymers (TDES-MIPs). A meticulous miniaturized solid-phase microextraction (mini-SPME) method followed by high-performance liquid chromatography (HPLC) were used for the optimal speciation of 3,4-dihydroxybenzoic acid (3,4-DHBA) in the needle of a syringe system with response surface methodology (RSM). Under the optimal conditions for the determination of 3,4-DHBA (amount of adsorbent (2 mg), sample volume (1 mL), cycles for adsorption and desorption (6)), the actual extraction amount was 8.46 μg g−1. The limits of detection (LODs, S/N = 3) for 3,4-DHBA in Ilex chinensis Sims were 0.26–0.31 μg mL−1, and the intra-day and inter-day precision (relative standard deviations, n = 4) after spiking with 5 μg mL−1, 100 μg mL−1, and 200 μg mL−1 were both less than 4.21%. The meticulous method (TDES-MIP-mini-SPME) combined with RSM offers a significant advance over existing methods, because of the meticulous operation and excellent selectivity of 3,4-DHBA from complex samples.

Open image in new windowGraphical abstract
Graphical abstract



中文翻译:

选择性提取3,4-二羟基苯甲酸。 冬青 使用三元深共熔溶剂基分子印迹聚合物通过精细的微固相微萃取进行模拟

在基于TDES的分子印迹聚合物(TDES-MIP)的合成中,将三元深共熔溶剂(TDES)用作模板和功能单体。细微的微型固相微萃取(mini-SPME)方法,然后用高效液相色谱(HPLC)用于注射器系统针头中3,4-二羟基苯甲酸(3,4-DHBA)的最佳形态使用响应面方法(RSM)。在确定3,4-DHBA的最佳条件下(吸附剂量(2 mg),样品量(1 mL),吸附和解吸循环(6)),实际提取量为8.46μgg -1冬青树Sims中3,4-DHBA的检出限(LODs,S / N = 3)为0.26-0.31μgmL -1和日内和日间精密度(相对标准偏差,Ñ 用5μg毫升尖峰之后= 4)-1,100微克毫升-1,和200μg毫升-1均小于4.21%。细致的方法(TDES-MIP-mini-SPME)与RSM相结合,比现有方法有了重大进步,这是因为细致的操作和对复杂样品中3,4-DHBA的优异选择性。

在新窗口中打开图像图形概要
图形概要

更新日期:2018-10-03
down
wechat
bug