We have prepared and evaluated larger format phage-bound epoxy-cryogel columns in order to increase the yield of bound target. Freezing thermograms showed that larger column formats (2.5–5 cm diameter) are not usable due to irregular polymerization phenomena. Preparing thin disks of 0.5 cm height with similar diameter proved to be an excellent alternative. Disks could be stacked and run in a chromatographic setup. In this way, we could increase the matrix volume, ligand-binding capacity, and finally the yield of bound target. By increasing the column volume about sevenfold, we observed a 12-fold increase of ligand density and a sevenfold increase in the yield of protein recovery in a column where phages were attached without spacer and a 10- to 34-fold increase in a spacer column, depending on the spacer used.

中文翻译：

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用于亲和色谱和生物技术应用的大幅面大孔冷冻凝胶盘的制备和表征

我们已经准备并评估了较大规格的噬菌体结合环氧冷凝胶色谱柱，以提高结合靶标的收率。冷冻热分析图显示，由于不规则的聚合现象，无法使用较大的色谱柱形式（直径2.5-5 cm）。事实证明，准备直径类似的0.5厘米高的薄盘是一种很好的选择。磁盘可以堆叠并以色谱设置运行。这样，我们可以增加基质体积，配体结合能力，最后增加结合靶标的产量。通过将色谱柱体积增加约7倍，我们观察到在没有间隔物的情况下连接噬菌体的色谱柱中配体密度增加了12倍，蛋白质回收率提高了7倍，而在间隔柱中增加了10到34倍，取决于所用的垫片。