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Noncovalent spin-labeling of RNA: the aptamer approach†
Chemical Communications ( IF 4.3 ) Pub Date : 2018-10-02 00:00:00 , DOI: 10.1039/c8cc05597a
Subham Saha 1, 2, 3 , Thilo Hetzke 4, 5, 6 , Thomas F. Prisner 4, 5, 6 , Snorri Th. Sigurdsson 1, 2, 3
Affiliation  

In the first example of site-directed spin-labeling of unmodified RNA, a pyrrolidine-nitroxide derivative of tetramethylrosamine (TMR) was shown to bind with high affinity to the malachite green (MG) aptamer, as determined by continuous-wave (CW) electron paramagnetic resonance (EPR), pulsed electron–electron double resonance (PELDOR) and fluorescence spectroscopies.

中文翻译:

RNA的非共价自旋标记:适体方法

在未修饰RNA的定点旋转标记的第一个例子中,四甲基罗莎明(TMR)的吡咯烷-硝基氧化物衍生物与孔雀石绿(MG)适体具有高亲和力,如通过连续波(CW)确定的那样电子顺磁共振(EPR),脉冲电子-电子双共振(PELDOR)和荧光光谱。
更新日期:2018-10-02
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