The ability to design detergents that are suitable for protein analysis by mass spectrometry (MS) represents an on-going challenge in the field of native MS. Desirable detergent characteristics include charge-reducing properties and low gas-phase stabilities of complexes formed with proteins. In this work, the gas-phase properties of oligoglycerol detergents (OGDs) are optimized by fine tuning of their molecular structure. Furthermore, a tandem mass spectrometry (MS/MS) approach is presented that estimates the gas-phase properties of detergents simply by studying the dissociation behaviour of protein-detergent complexes (PDCs) formed with the soluble protein β-lactoglobulin (BLG). Graphical Abstract ᅟ.

中文翻译：

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探索树突状低聚甘油洗涤剂在蛋白质质谱中的潜力。

设计适用于通过质谱（MS）进行蛋白质分析的去污剂的能力代表了天然MS领域的持续挑战。理想的去污剂特性包括与蛋白质形成的复合物的电荷减少特性和低气相稳定性。在这项工作中，通过微调分子结构来优化低聚甘油洗涤剂（OGD）的气相性能。此外，提出了一种串联质谱（MS / MS）方法，该方法可以简单地通过研究与可溶性蛋白β-乳球蛋白（BLG）形成的蛋白洗涤剂复合物（PDC）的解离行为来估算洗涤剂的气相性质。图形摘要ᅟ。