The fecal morphology, defecation frequency, bowel function, intestinal motility, and fecal bacterial composition were evaluated to investigate the laxative effect of probiotic chocolate containing Streptococcus thermophilus MG510 and Lactobacillus plantarum LRCC5193 (LYC) on loperamide-induced constipated rats. Daily oral administration of LYC in constipated rats for two weeks was shown to significantly increase (n = 14) the defecation frequency, fecal moisture content, and relative abundance of fecal Lactobacillus and Faecalibacterium prausnitzii. Moreover, histological analysis of the distal colon of constipated rats revealed that LYC treatment can also increase the thickness of the colonic mucosa and muscle layers, and crypt of Lieberkühn. LYC also significantly increased (n = 5) the intestinal motility and modulated (n = 9) mRNA expression levels of colonic ZO-1 and Cldn-1 in the constipated rats. Altogether, these results demonstrate that probiotic chocolate has potential as a dietary adjunct for the treatment of constipation.

评估粪便的形态，排便频率，肠功能，肠蠕动和粪便细菌组成，以研究含有嗜热链球菌MG510和植物乳杆菌LRCC5193（LYC）的益生菌巧克力对洛哌丁胺诱发的便秘大鼠的通便作用。在便秘大鼠中每日口服LYC两周可显着增加（n = 14）排便频率，粪便水分含量以及粪便乳酸杆菌和费氏杆状杆菌的相对丰度。此外，对便秘大鼠远端结肠的组织学分析显示，LYC治疗还可以增加结肠粘膜和肌肉层的厚度以及Lieberkühn的隐窝。LYC还显着提高了便秘大鼠肠运动性（n = 5）和结肠ZO-1和Cldn-1的mRNA表达水平（n = 9）。总而言之，这些结果表明益生菌巧克力具有作为饮食辅助剂治疗便秘的潜力。