Simple tuning of a host:guest pair allows selective sensing of different peptide modifications, exploiting orthogonal recognition mechanisms. Excellent selectivity for either lysine trimethylations or alcohol phosphorylations is possible by simply varying the fluorophore guest. The phosphorylation sensor can be modulated by the presence of small (μM) concentrations of metal ions, allowing array-based sensing. Phosphorylation at serine, threonine, and tyrosine can be selectively sensed via discriminant analysis. The phosphopeptide sensing is effective in the presence of small-molecule phosphates such as ATP, which in turn enables the sensor to be employed in continuous optical assays of both serine kinase and tyrosine phosphatase activity. The activity of multiple different kinases can be monitored, and the sensor is capable of detecting the phosphorylation of peptides containing multiple different modifications, including lysine methylations and acetylation. A single deep cavitand can be used as a "one size fits all" sensor that can selectively detect multiple different modifications to oligopeptides, as well as monitoring the function of their post-translational modification writer and eraser enzymes in complex systems.

中文翻译：

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使用超分子串联分析选择性检测磷酸化肽并监测激酶和磷酸酶活性

宿主：客体对的简单调整允许利用正交识别机制选择性感知不同的肽修饰。通过简单地改变荧光团客体，可以对赖氨酸三甲基化或醇磷酸化具有出色的选择性。磷酸化传感器可以通过小 (μM) 浓度的金属离子的存在进行调节，从而实现基于阵列的传感。可以通过判别分析选择性地检测丝氨酸、苏氨酸和酪氨酸的磷酸化。磷酸肽传感在小分子磷酸盐（如 ATP）存在时有效，这反过来又使传感器能够用于丝氨酸激酶和酪氨酸磷酸酶活性的连续光学测定。可以监测多种不同激酶的活性，该传感器能够检测含有多种不同修饰的肽的磷酸化，包括赖氨酸甲基化和乙酰化。单个深空腔可用作“一刀切”传感器，可以选择性地检测寡肽的多种不同修饰，并监测复杂系统中其翻译后修饰写入酶和擦除酶的功能。