The overwhelming response towards algal biodiesel production has been well-recognized recently as a sustainable alternative to conventional fuels. Most microalgae cannot grow well at acidic pH. The present study, therefore, investigated whether non-acidophilic microalgae Desmodesmus sp. MAS1 and Heterochlorella sp. MAS3 can be acclimated to extreme-acidic pH for sustainable production of biomass and biodiesel. Growth analysis indicated that both the microalgal strains possessed a passive uptake of CO₂ at pH 3.0 with biomass production of 0.25 g dry wt. L⁻¹ in Desmodemus sp. and 0.45 g dry wt. L⁻¹ in Heterochlorella sp.. Flow-cytometry analysis for reactive oxygen species, membrane permeability and neutral-lipids revealed the capabilities of both strains to adapt to the stress imposed by acidic pH. Lipid production was doubled in both the strains when grown at pH 3.0. In-situ transesterification of biomass resulted in 13–15% FAME yield in the selected microalgae, indicating their great potential in biofuel production.

近来，藻类生物柴油生产的反应热烈，已被公认是传统燃料的可持续替代品。大多数微藻在酸性pH下不能很好地生长。因此，本研究调查了非嗜酸性微藻Desmodesmus sp。MAS1和Heterochlorella sp。MAS3可以适应极端酸性的pH，以可持续地生产生物质和生物柴油。生长分析表明，两种微藻菌株在pH 3.0时均具有被动吸收CO _2的能力，而生物质的产量为0.25 g干重。大号^-1在Desmodemus SP。和0.45克干重。大号^-1在Heterochlorella流式细胞仪分析活性氧，膜通透性和中性脂质，揭示了两种菌株适应酸性pH所施加压力的能力。在pH 3.0下生长时，两种菌株的脂质产量均增加了一倍。生物质的原位酯交换反应在选定的微藻中产生了13–15％的FAME收率，表明它们在生物燃料生产中具有巨大的潜力。