AbstractThe authors report on a one-pot approach for synthesizing highly fluorescent protamine-stabilized gold nanoclusters. These are shown to be a viable nanoprobe for selective and sensitive fluorometric determination of lead(II) via quenching of fluorescence via Pb(II)-Au(I) interaction. Under optimized conditions, fluorescence measured at excitation/emission peaks of 300/599 nm drops in the 80 nM–15 μM lead(II) concentration range. The detection limit is 24 nM, and relative standard deviations (for n = 11) at concentrations of 0.10, 4.0 and 15 μM are 1.6, 2.5 and 1.9%, respectively. The relative recoveries of added lead(II) in the water samples ranged from 97.9 ± 2.29% to 101.2 ± 1.83%. Graphical abstractLead(II) ions are found to be able to selectively and sensitively quench the fluorescence of the protamine-gold nanoclusters (PRT-AuNCs). Thereby, an inexpensive, selective and sensitive lead(II) assay was established.

中文翻译：

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鱼精蛋白稳定的金纳米团簇作为通过 Pb(II)-Au(I) 相互作用检测铅 (II) 的荧光纳米探针

摘要作者报告了一种用于合成高荧光鱼精蛋白稳定的金纳米团簇的一锅法。这些被证明是一种可行的纳米探针，用于通过 Pb(II)-Au(I) 相互作用淬灭荧光来选择性和灵敏地荧光测定铅 (II)。在优化条件下，在 300/599 nm 激发/发射峰处测得的荧光在 80 nM–15 μM 铅 (II) 浓度范围内下降。检测限为 24 nM，浓度为 0.10、4.0 和 15 μM 时的相对标准偏差（n = 11）分别为 1.6、2.5 和 1.9%。水样中添加的铅 (II) 的相对回收率为 97.9 ± 2.29% 至 101.2 ± 1.83%。图形摘要铅 (II) 离子被发现能够选择性和灵敏地淬灭鱼精蛋白金纳米团簇 (PRT-AuNCs) 的荧光。