The objective of our work was to investigate the effects of different types of nanoparticles on endothelial (HUVEC) and monocytic cell functions. We prepared and tested 14 different nanosystems comprising liposomes, lipid nanoparticles, polymer, and iron oxide nanoparticles. Some of the tested nanosystems contained targeting, therapeutic, or contrast agent(s). The effect of particles (0–400 µg/mL) on endothelial-monocytic cell interactions in response to TNF-α was investigated using an arterial bifurcation model and dynamic monocyte adhesion assay. Spontaneous HUVEC migration (0–100 µg/mL nanoparticles) and chemotaxis of monocytic cells towards MCP-1 in presence of particles (0–400 µg/mL) were determined using a barrier assay and a modified Boyden chamber assay, respectively. Lipid nanoparticles dose-dependently reduced monocytic cell chemotaxis and adhesion to activated HUVECs. Liposomal nanoparticles had little effect on cell migration, but one formulation induced monocytic cell recruitment by HUVECs under non-uniform shear stress by about 50%. Fucoidan-coated polymer nanoparticles (25–50 µg/mL) inhibited HUVEC migration and monocytic cell chemotaxis, and had a suppressive effect on monocytic cell recruitment under non-uniform shear stress. No significant effects of iron oxide nanoparticles on monocytic cell recruitment were observed except lauric acid and human albumin-coated particles which increased endothelial-monocytic interactions by 60–70%. Some of the iron oxide nanoparticles inhibited HUVEC migration and monocytic cell chemotaxis. These nanoparticle-induced effects are of importance for vascular cell biology and function and must be considered before the potential clinical use of some of the analyzed nanosystems in cardiovascular applications.

中文翻译：

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纳米系统对人内皮细胞和单核细胞功能影响的比较分析

我们工作的目的是研究不同类型的纳米颗粒对内皮（HUVEC）和单核细胞功能的影响。我们准备并测试了14种不同的纳米系统，包括脂质体，脂质纳米颗粒，聚合物和氧化铁纳米颗粒。一些测试的纳米系统包含靶向剂，治疗剂或造影剂。使用动脉分叉模型和动态单核细胞粘附试验研究了颗粒（0–400 µg / mL）对TNF-α响应的内皮-单核细胞相互作用的影响。HUVEC的自发迁移（0–100 µg / mL纳米颗粒）和存在颗粒（0–400 µg / mL）的单核细胞趋于MCP-1的趋化性分别通过屏障测定和改良的博登室测定来确定。脂质纳米颗粒剂量依赖性地减少了单核细胞的趋化性和对活化HUVEC的粘附。脂质体纳米颗粒对细胞迁移的影响很小，但是一种制剂在不均匀的剪切应力下诱导HUVEC募集单核细胞约50％。涂有岩藻依丹的聚合物纳米粒子（25–50 µg / mL）抑制HUVEC迁移和单核细胞趋化性，并且在非均匀剪切应力下对单核细胞募集具有抑制作用。除月桂酸和人白蛋白涂层的颗粒使内皮-单核细胞的相互作用增加了60-70％外，没有观察到氧化铁纳米颗粒对单核细胞募集的显着影响。一些氧化铁纳米粒子抑制HUVEC迁移和单核细胞趋化性。