Passion fruit-like nano-architectures (NAs) are all-in-one platforms of increasing interest for the translation of metal nanoparticles into clinics. NAs are nature-inspired disassembling inorganic theranostics, which jointly combine most of the appealing behaviors of noble metal nanoparticles with their potential organism excretion. Despite their unique and promising properties, NAs in vivo interactions and potential adverse effects have not yet been investigated. In this study, we employ zebrafish (Danio Rerio) to assess the development toxicity of NAs as well as their uptake and bioaccumulation at different stages of growth. The evaluation of multiple endpoints related to the toxicity clearly indicates that NAs do not induce mortality, developmental defects, or alterations on the hatching rate and behavior of zebrafish. Moreover, the analysis of nanostructures uptake and biodistribution demonstrates that NAs are successfully internalized and present a specific localization. Overall, our results demonstrate that NAs are able to pass through the embryos chorion and accumulate in specific tissues, exhibiting an impressive biocompatibility.

中文翻译：

---

斑马鱼中百香果状纳米结构的生物分布和生物相容性

百香果状的纳米结构（NAs）是将金属纳米颗粒翻译到临床中越来越受到关注的多合一平台。NAs是受自然启发的可分解的无机治疗学，将贵金属纳米颗粒的大多数吸引人的行为与其潜在的生物体排泄物结合在一起。尽管它们具有独特和有希望的性质，但尚未研究NA在体内的相互作用和潜在的不良反应。在这项研究中，我们使用斑马鱼（Danio Rerio）评估NAs的发育毒性以及其在不同生长阶段的摄取和生物蓄积。对与毒性有关的多个终点的评估清楚地表明，NAs不会导致死亡率，发育缺陷或斑马鱼的孵化率和行为改变。此外，对纳米结构吸收和生物分布的分析表明，NAs已成功内化并呈现出特定的定位。总体而言，我们的结果表明，NAs能够穿过绒毛膜并积累在特定组织中，表现出令人印象深刻的生物相容性。