Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Living Nanospear for Near-Field Optical Probing
ACS Nano ( IF 15.8 ) Pub Date : 2018-09-28 00:00:00 , DOI: 10.1021/acsnano.8b05235 Yuchao Li 1 , Hongbao Xin 1 , Yao Zhang 1 , Hongxiang Lei 2 , Tianhang Zhang 3, 4 , Huapeng Ye 4 , Juan Jose Saenz 5, 6 , Cheng-Wei Qiu 3, 4 , Baojun Li 1
ACS Nano ( IF 15.8 ) Pub Date : 2018-09-28 00:00:00 , DOI: 10.1021/acsnano.8b05235 Yuchao Li 1 , Hongbao Xin 1 , Yao Zhang 1 , Hongxiang Lei 2 , Tianhang Zhang 3, 4 , Huapeng Ye 4 , Juan Jose Saenz 5, 6 , Cheng-Wei Qiu 3, 4 , Baojun Li 1
Affiliation
|
Optical nanoprobes, designed to emit or collect light in the close proximity of a sample, have been extensively used to sense and image at nanometer resolution. However, the available nanoprobes, constructed from artificial materials, are incompatible and invasive when interfacing with biological systems. In this work, we report a fully biocompatible nanoprobe for subwavelength probing of localized fluorescence from leukemia single-cells in human blood. The bioprobe is built on a tapered fiber tip apex by optical trapping of a yeast cell (1.4 μm radius) and a chain of Lactobacillus acidophilus cells (2 μm length and 200 nm radius), which act as a high-aspect-ratio nanospear. Light propagating along the bionanospear can be focused into a spot with a full width at half-maximum (fwhm) of 190 nm on the surface of single cells. Fluorescence signals are detected in real time at subwavelength spatial resolution. These noninvasive and biocompatible optical probes will find applications in imaging and manipulation of biospecimens.
中文翻译：
用于近场光学探测的活性纳米矛
设计用于在样品附近发射或收集光的光学纳米探针已被广泛用于以纳米分辨率感应和成像。然而，当与生物系统连接时，由人造材料制成的可用纳米探针是不相容的且具有侵入性。在这项工作中，我们报告了一种完全生物相容的纳米探针，用于亚波长探测人血中白血病单细胞的局部荧光。通过光学捕获酵母细胞（半径为1.4μm）和嗜酸乳杆菌链，将生物探针构建在锥形纤维尖端的尖端上细胞（长度为2μm，半径为200 nm），用作高长宽比的纳米矛。沿仿生矛传播的光可以聚焦到单个细胞表面上半峰全宽（wwhm）处最大190 nm的光斑中。以亚波长空间分辨率实时检测荧光信号。这些无创且生物相容的光学探针将在生物标本的成像和操作中找到应用。
更新日期：2018-09-28
中文翻译：
用于近场光学探测的活性纳米矛
设计用于在样品附近发射或收集光的光学纳米探针已被广泛用于以纳米分辨率感应和成像。然而，当与生物系统连接时，由人造材料制成的可用纳米探针是不相容的且具有侵入性。在这项工作中，我们报告了一种完全生物相容的纳米探针，用于亚波长探测人血中白血病单细胞的局部荧光。通过光学捕获酵母细胞（半径为1.4μm）和嗜酸乳杆菌链，将生物探针构建在锥形纤维尖端的尖端上细胞（长度为2μm，半径为200 nm），用作高长宽比的纳米矛。沿仿生矛传播的光可以聚焦到单个细胞表面上半峰全宽（wwhm）处最大190 nm的光斑中。以亚波长空间分辨率实时检测荧光信号。这些无创且生物相容的光学探针将在生物标本的成像和操作中找到应用。
京公网安备 11010802027423号