The infections caused by gram-positive bacteria (G⁺) is a major threat to public health due to their high morbidity and mortality, and the identification of G⁺ with high sensitivity and selectivity is urgently desirable. In this paper, a probe with aggregation-induced emission (AIE) characteristic is developed and used to discriminate G⁺ from other bacteria and fungus with excellent selectivity. Thanks for its AIE feature, this probe shows intense fluorescence when interacted with G⁺, and the signal can last for 24 h after incubating with S. aureus, which is benefit for long term tracing of translocation process with high contrast. Moreover, this probe can be used to visualize the infection processes of G⁺ towards mammalian cells, which provides valuable information for understanding the therapeutic mechanism.

由革兰氏阳性细菌（G ⁺）引起的感染由于其高发病率和死亡率而成为对公共卫生的主要威胁，因此迫切需要以高灵敏度和高选择性来鉴定G ⁺。本文开发了一种具有聚集诱导发射（AIE）特性的探针，并以优异的选择性将G ⁺与其他细菌和真菌区分开。由于其AIE功能，该探针与G ⁺相互作用时显示强烈的荧光，并且与金黄色葡萄球菌孵育后该信号可以持续24小时，这对于以高对比度长期定位易位过程很有帮助。此外，该探针可用于可视化G ⁺对哺乳动物细胞的感染过程，这为了解治疗机理提供了有价值的信息。