Osmotic-Stress-Mediated Control of Membrane Permeability of Polymeric Microcapsules,Chemistry of Materials

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Osmotic-Stress-Mediated Control of Membrane Permeability of Polymeric Microcapsules
Chemistry of Materials ( IF 7.2 ) Pub Date : 2018-09-27 00:00:00 , DOI: 10.1021/acs.chemmater.8b03230
Sangmin Lee ₁ , Tae Yong Lee ₁ , Dong Jae Kim ₁ , Bomi Kim ₁ , Shin-Hyun Kim ₁

Affiliation

Microcapsules with regular pore size can provide size-selective permeation, which is promising for immunoisolation of cells, protection of enzymes or catalysts, and development of capsule-type sensors. However, conventional approaches have limited biocompatibility or poor dispersion stability of encapsulants. Here, we suggest a simple yet pragmatic method to produce semipermeable microcapsules using osmotic stress. With a capillary microfluidic device, monodisperse microcapsules with ultrathin polymer membranes are prepared by double-emulsion templating. The microcapsules are subjected to a hypotonic condition, by which water is pumped in imposing a tensile stress on the membrane. The osmotic stress initiates cracks at weak spots. As cracks propagate, the pressure gradually reduces as ions diffuse through them, finally resulting in a finite width of cracks. The final width can be controlled from 5 to 10 nm using an initial osmotic pressure of 230 to 690 kPa, enabling fine adjustment of the cutoff threshold of permeation. This osmotic-pressure-mediated control is highly compatible with delicate biological molecules and colloidal dispersions as no etching chemicals are required to form pores. Taking advantage of this method, we demonstrate a capsule-type molecular sensor based on surface-enhanced Raman scattering that obviates pretreatment of samples because the membrane allows the entrance of small target molecules while blocking the large adhesive proteins.

中文翻译：

渗透-压力介导的聚合物微囊膜通透性的控制

具有规则孔径的微胶囊可以提供尺寸选择性的渗透，这有望用于细胞的免疫分离，酶或催化剂的保护以及胶囊型传感器的开发。但是，常规方法的生物相容性有限或密封剂的分散稳定性差。在这里，我们建议一种简单而实用的方法来利用渗透压生产半透性微胶囊。使用毛细管微流体装置，通过双乳液模板制备具有超薄聚合物膜的单分散微胶囊。使微胶囊处于低渗状态，通过泵送水以在膜上施加拉伸应力。渗透应力在弱点处引发裂纹。随着裂纹的传播，离子逐渐扩散，压力逐渐降低，最终导致有限的裂缝宽度。使用230至690 kPa的初始渗透压，可以将最终宽度控制在5至10 nm之间，从而可以精确调节渗透的截止阈值。这种渗透压介导的控制与精细的生物分子和胶体分散体高度兼容，因为不需要蚀刻化学品即可形成孔。利用这种方法，我们展示了一种基于表面增强拉曼散射的胶囊型分子传感器，该传感器无需进行样品预处理，因为该膜允许小目标分子进入，同时阻止了大的粘附蛋白。这种渗透压介导的控制与精细的生物分子和胶体分散体高度兼容，因为不需要蚀刻化学品即可形成孔。利用这种方法，我们展示了一种基于表面增强拉曼散射的胶囊型分子传感器，该传感器无需进行样品预处理，因为该膜允许小目标分子进入，同时阻止了大的粘附蛋白。这种渗透压介导的控制与精细的生物分子和胶体分散体高度兼容，因为不需要蚀刻化学品即可形成孔。利用这种方法，我们展示了一种基于表面增强拉曼散射的胶囊型分子传感器，该传感器无需进行样品预处理，因为该膜允许小目标分子进入，同时阻止了大的粘附蛋白。

更新日期：2018-09-27

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