An enhanced enzymatic reaction using a triphase system based on superhydrophobic mesoporous nanowire arrays.,Nanoscale Horizons

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An enhanced enzymatic reaction using a triphase system based on superhydrophobic mesoporous nanowire arrays.
Nanoscale Horizons ( IF 8.0 ) Pub Date : 2018-10-05 , DOI: 10.1039/c8nh00184g
Fengying Guan ₁ , Jun Zhang , Heming Tang , Liping Chen , Xinjian Feng

Affiliation

Gaseous reactants play a key role in a wide range of biocatalytic reactions, however reaction kinetics are generally limited by the slow mass transport of gases (typically oxygen) in or through aqueous solutions. Inspired by the morphologies of natural non-wetting surfaces, herein we address this limitation by developing a triphase reaction system possessing a triphase gas-solid-liquid interface. As a proof of concept, we study the kinetics of glucose oxidase (GOx) catalyzed reactions using a triphase system fabricated by layering GOx upon superhydrophobic mesoporous ZnO nanowire arrays through which oxygen, needed for the enzymatic reaction, is supplied directly from the atmosphere to the liquid-solid interface. We find that the enzymatic reaction rate is enhanced by a factor of 30 over that obtained from a conventional diphase system where oxygen is supplied through and from the liquid. The triphase system offers the opportunity to develop high performance bioassay systems, serving as an enabling platform for addressing challenges posed by gas-deficit kinetics.

中文翻译：

使用基于超疏水介孔纳米线阵列的三相系统增强的酶促反应。

气态反应物在广泛的生物催化反应中起着关键作用，但是反应动力学通常受到气体（通常为氧气）在水溶液中或通过水溶液的缓慢质量传输的限制。受自然非润湿表面形态的启发，在此我们通过开发具有三相气-固-液界面的三相反应系统来解决这一局限性。作为概念的证明，我们使用三相系统研究葡萄糖氧化酶（GOx）催化的反应动力学，该三相系统是通过在超疏水介孔ZnO纳米线阵列上层叠GOx制成的，酶催化反应所需的氧气通过该系统直接从大气中提供给液固界面。我们发现，酶促反应速率比常规的双相体系（通过液体从中供氧）提供的酶促反应速率提高了30倍。三相系统为开发高性能生物测定系统提供了机会，可作为应对气体亏空动力学带来的挑战的支持平台。

更新日期：2018-09-27

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