Dragon blood has been used in wound treatment for many years and can be obtained from several distinct plant species. Dracorhodin, the active substituent of dragon blood, is a characteristic compound of the palm tree, Daemonorops draco. At present, the only method to evaluate the quality of commercial dragon blood samples is a HPLC method which determines the amount of dracorhodin in a dragon blood sample. In this study, we used zebrafish embryos as a platform to demonstrate the in vivo pro-angiogenic activity of dracorhodin perchlorate, the chemically synthesized analog of dracorhodin. By using this platform, three different commercial dragon blood samples were also examined. Our results clearly show that even though the commercial dragon blood samples had similar amounts of dracorhodin, they showed highly variable biological activity, such as pro-angiogenic effects and toxicity. In short, an in vivo activity assay platform for rapidly examining the biological activity of commercial dragon blood samples was successfully established here, which complements the current HPLC-based assay method.

中文翻译：

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高氯酸龙血红素在斑马鱼胚胎中的体内促血管生成作用：商业龙血样本的新型生物活性评估平台

龙血多年来一直用于伤口治疗，可以从几种不同的植物中获得。Dracorhodin 是龙血的活性取代基，是棕榈树 Daemonorops draco 的特征化合物。目前，评估商业龙血样本质量的唯一方法是 HPLC 方法，该方法确定龙血样本中的 dracorhodin 含量。在这项研究中，我们使用斑马鱼胚胎作为平台来展示 dracorhodin perchlorate（一种化学合成的 dracorhodin 类似物）的体内促血管生成活性。通过使用该平台，还检查了三种不同的商业龙血样本。我们的结果清楚地表明，即使商业龙血样本中的龙血红素含量相似，它们也显示出高度可变的生物活性，例如促血管生成作用和毒性。总之，这里成功地建立了一个快速检测商业龙血样本生物活性的体内活性测定平台，它补充了当前基于高效液相色谱的测定方法。