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Characterization of polyphenols in Australian sweet lupin (Lupinus angustifolius) seed coat by HPLC-DAD-ESI-MS/MS
Food Research International ( IF 7.0 ) Pub Date : 2018-09-28 , DOI: 10.1016/j.foodres.2018.09.061
Liezhou Zhong , Gangcheng Wu , Zhongxiang Fang , Mark L. Wahlqvist , Jonathan M. Hodgson , Michael W. Clarke , Edwin Junaldi , Stuart K. Johnson

Seeds of the legume lupin (Lupinus spp.) are becoming increasingly important as human food. The seed coat, at ~25% of the whole seed of Lupinus angustifolius (Australian sweet lupin, ASL), is the main by-product of lupin kernel flour production. The primary market for lupin seed coat is low value feed with very limited use in foods. In this study, seed coats of six ASL commercial varieties from two growing sites were sampled for identification and quantification of polyphenols using a high-performance liquid chromatography (HPLC) with diode array detector (DAD) and coupled with a triple quadrupole mass spectrometer which equipped with electrospray ionization source (ESI-MS/MS). Three flavones (apigenin-7-O-β-apiofuranosyl-6,8-di-C-β-glucopyranoside, vicenin 2, and apigenin-7-O-β-glucopyranoside), one isoflavone (genistein) and one dihydroflavonol derivative (aromadendrin-6-C-β-d-glucopyranosyl-7-O-[β-D-apiofuranosyl-(1 → 2)]-O-β-D-glucopyranoside), and several hydroxybenzoic and hydroxycinnamic acid derivatives were identified. Considerable variations in levels of individual polyphenols were found but apigenin-7-O-β-apiofuranosyl-6,8-di-C-β-glucopyranoside was the predominant polyphenol in all samples accounting for 73.08–82.89% of the total free polyphenols. These results suggest that ASL seed coat could be valuable dietary source of polyphenols.



中文翻译:

在澳大利亚甜羽扇豆(多酚的表征羽扇豆通过HPLC-DAD-ESI-MS)种皮/ MS

豆科植物羽扇豆(羽扇豆属)的种子作为人类食物正变得越来越重要。种皮约占羽扇豆Lupinus angustifolius)(澳大利亚甜羽扇豆,ASL)种子总数的25%,是羽扇豆仁粉生产的主要副产品。羽扇豆种皮的主要市场是低价值饲料,其在食品中的用途非常有限。在这项研究中,使用两个带有二极管阵列检测器(DAD)的高效液相色谱(HPLC)并配备了配备了三重四极杆质谱仪的高效液相色谱(HPLC),对来自两个生长地点的六个ASL商业品种的种皮进行了采样,以鉴定和定量多酚。带有电喷雾电离源(ESI-MS / MS)。三个黄酮(芹菜素-7- O -β-apiofuranosyl-6,8-di- C-β-吡喃葡萄糖苷,总蛋白2和芹菜素7- O -β-吡喃葡萄糖苷),一种异黄酮(染料木黄酮)和一种二氢黄酮醇衍生物(芳香金属烯酮-6- C - β- d-吡喃葡萄糖基-7- O- [β-D -apiofuranosyl-((1→2)]- O - β -D- pyramyranoside ),并鉴定了几种羟基苯甲酸和羟基肉桂酸衍生物。发现各个多酚的水平存在很大差异,但芹菜素-7- O -β-apiofuranosyl-6,8-di- C -β-吡喃葡萄糖苷是所有样品中的主要多酚,占游离多酚总量的73.08-82.89%。这些结果表明,ASL种皮可能是多酚的重要膳食来源。

更新日期:2018-09-28
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