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Electrically Controllable Single-Point Covalent Functionalization of Spin-Cast Carbon-Nanotube Field-Effect Transistor Arrays
ACS Nano ( IF 15.8 ) Pub Date : 2018-09-27 00:00:00 , DOI: 10.1021/acsnano.8b03073 Yoonhee Lee 1 , Scott M. Trocchia 1 , Steven B. Warren 2 , Erik F. Young 1 , Sefi Vernick 3 , Kenneth L. Shepard 1
ACS Nano ( IF 15.8 ) Pub Date : 2018-09-27 00:00:00 , DOI: 10.1021/acsnano.8b03073 Yoonhee Lee 1 , Scott M. Trocchia 1 , Steven B. Warren 2 , Erik F. Young 1 , Sefi Vernick 3 , Kenneth L. Shepard 1
Affiliation
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Single-point-functionalized carbon-nanotube field-effect transistors (CNTFETs) have been used to sense conformational changes and binding events in protein and nucleic acid structures from intrinsic molecular charge. The key to utilizing these devices as single-molecule sensors is the ability to attach a single probe molecule to an individual device. In contrast, with noncovalent attachment approaches such as those based on van der Waals interactions, covalent attachment approaches generally deliver higher stability but have traditionally been more difficult to control, resulting in low yield. Here, we present a single-point-functionalization method for CNTFET arrays based on electrochemical control of a diazonium reaction to create sp3 defects, combined with a scalable spin-casting method for fabricating large arrays of devices on arbitrary substrates. Attachment of probe DNA to the functionalized device enables single-molecule detection of DNA hybridization with complementary target, verifying the single-point functionalization. Overall, this method enables single-point defect generation with 80% yield.
中文翻译：
自旋铸造碳纳米管场效应晶体管阵列的电控单点共价功能化
单点功能化碳纳米管场效应晶体管（CNTFET）已用于从固有分子电荷感测蛋白质和核酸结构中的构象变化和结合事件。将这些设备用作单分子传感器的关键是将单个探针分子连接到单个设备的能力。相反，对于非共价连接方法，例如基于范德华相互作用的非共价连接方法，共价连接方法通常可提供更高的稳定性，但传统上较难控制，导致产量低。在这里，我们提出了基于重氮反应的电化学控制以创建sp 3的CNTFET阵列的单点功能化方法缺陷，结合可伸缩的旋转浇铸方法，可在任意基板上制造大型器件阵列。探针DNA与功能化设备的连接可实现与互补靶标的DNA杂交的单分子检测，从而验证了单点功能化。总的来说，这种方法能够以80％的合格率产生单点缺陷。
更新日期：2018-09-27
中文翻译：
自旋铸造碳纳米管场效应晶体管阵列的电控单点共价功能化
单点功能化碳纳米管场效应晶体管（CNTFET）已用于从固有分子电荷感测蛋白质和核酸结构中的构象变化和结合事件。将这些设备用作单分子传感器的关键是将单个探针分子连接到单个设备的能力。相反，对于非共价连接方法，例如基于范德华相互作用的非共价连接方法，共价连接方法通常可提供更高的稳定性，但传统上较难控制，导致产量低。在这里，我们提出了基于重氮反应的电化学控制以创建sp 3的CNTFET阵列的单点功能化方法缺陷，结合可伸缩的旋转浇铸方法，可在任意基板上制造大型器件阵列。探针DNA与功能化设备的连接可实现与互补靶标的DNA杂交的单分子检测，从而验证了单点功能化。总的来说，这种方法能够以80％的合格率产生单点缺陷。
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