Simultaneous and Selective Imaging of Dopamine and Glutamate Using an Enzyme‐modified Large‐scale Integration (LSI)‐based Amperometric Electrochemical Device,Electroanalysis

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Simultaneous and Selective Imaging of Dopamine and Glutamate Using an Enzyme‐modified Large‐scale Integration (LSI)‐based Amperometric Electrochemical Device
Electroanalysis ( IF 2.7 ) Pub Date : 2018-09-27 , DOI: 10.1002/elan.201800386
Hiroya Abe ₁ , Tomoki Iwama ₁ , Hiroshi Yabu ₂ , Kosuke Ino ₃ , Kumi Y. Inoue ₁ , Atsushi Suda ₄ , Ryota Kunikata ₄ , Masahki Matsudaira ₅ , Tomokazu Matsue ₁

Affiliation

Since neurotransmitters play critical roles in nervous systems, the imaging techniques that can visualize them are needed. This communication describes the development of a method for simultaneous imaging of dopamine (DA) and glutamate (Glu), typical neurotransmitters, using enzyme‐modified large‐scale integration (LSI)‐based amperometric devices, referred to “Bio‐LSI”. The DA was detected by using direct electrochemical oxidation while Glu was measured by using an indirect electrochemical reaction involving glutamate oxidase (GluOx)/horseradish peroxidase (HRP) and osmium (Os) polymer‐modified electrodes. The Os‐HRP polymer was modified on designated sensor electrodes through electrodeposition. The selective and simultaneous imaging of neurotransmitters such as DA and Glu was achieved by applying the enzyme‐modified Bio‐LSI.

中文翻译：

使用基于酶修饰的大规模集成（LSI）的安培电化学设备同时和选择性地对多巴胺和谷氨酸成像

由于神经递质在神经系统中起关键作用，因此需要能使它们可视化的成像技术。本通讯介绍了使用基于酶修饰的大规模集成（LSI）的安培计设备（称为“ Bio-LSI”）对典型的神经递质多巴胺（DA）和谷氨酸（Glu）进行同时成像的方法的开发。DA是通过直接电化学氧化来检测的，而Glu是通过涉及谷氨酸氧化酶（GluOx）/辣根过氧化物酶（HRP）和（Os）聚合物修饰的电极的间接电化学反应来测量的。Os-HRP聚合物通过电沉积在指定的传感器电极上进行了改性。通过应用酶修饰的Bio-LSI，可以同时对DA和Glu等神经递质进行选择性成像。

更新日期：2018-09-27

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