The emerging Fusarium mycotoxins beauvericin (BEA) and enniatin (ENN) A, ENN A1, ENN B and ENN B1 gain increasing interest due to their highly prevalent contamination of cereals and cereal products. After oral intake, the gastro-intestinal tract is the first possible site of interaction. In the present in vitro study, the relative cytotoxicity of these mycotoxins towards proliferating and differentiated intestinal porcine epithelial cells of the jejunum (IPEC-J2) was evaluated using flow cytometric viability analysis. IPEC-J2 cells showed the highest sensitivity to BEA and ENN A. In proliferating cells, incubation for 24h with 10 μM BEA caused complete disruption, while the viability percentage declined to 32% after 24h of incubation with 10 μM ENN A. ENN A1 and ENN B1 were less cytotoxic with 87% and 93% viable cells after 24h of incubation with 10 μM ENN A1 and B1, respectively. ENN B was the least cytotoxic since incubation at concentrations up to 100 μM resulted in 83% viable proliferating cells. The same trend was observed for differentiated cells. The limited in vitro cytotoxic effect of ENN B on intestinal cells corroborates previous in vivo findings in broiler chicken in which dietary ENN B had minimal effect on intestinal morphometry.

新兴的镰刀菌真菌毒素金黄色葡萄球菌（BEA）和恩尼汀（ENN）A，ENN A1，ENN B和ENN B1由于对谷物和谷物产品的高度污染而受到越来越多的关注。口服后，胃肠道是第一个可能的相互作用部位。在目前的体外在这项研究中，使用流式细胞仪活力分析评估了这些真菌毒素对空肠增生和分化的肠道猪上皮细胞（IPEC-J2）的相对细胞毒性。IPEC-J2细胞对BEA和ENN A的敏感性最高。在增殖细胞中，与10μMBEA一起孵育24h导致完全破坏，而与10μMENN A一起孵育24h后，存活率下降至32％。在分别与10μMENN A1和B1孵育24小时后，ENN B1对87％和93％的活细胞的细胞毒性较小。ENN B具有最小的细胞毒性，因为在浓度高达100μM的条件下孵育可产生83％的增殖细胞。对于分化的细胞观察到相同的趋势。有限的体外ENN B对肠道细胞的细胞毒性作用证实了肉仔鸡先前的体内发现，其中日粮ENN B对肠道形态的影响极小。