(S)-MeO-BIPHEP-metal complexes were firstly used as chiral extractants to separate amino acid enantiomers. Six amino acid enantiomers were employed to evaluate the stereoselectivities of (S)-MeO-BIPHEP-metal complexes. The results revealed that (S)-MeO-BIPHEP-Cu complex exhibited good abilities to separate phenylalanine (Phe), phenylglycine (Pheg), tyrosine (Tyr) and 3-chloro-phenylglycine (Cpheg) enantiomers with operational enantioselectivities (α) were 2.56, 2.24, 4.22 and 1.81, respectively. While (S)-MeO-BIPHEP-Pd complex exhibited good ability to separate 4-nitro-phenylalanine (Nphe) with operational enantioselectivity (α) was 3.87. All of (S)-MeO-BIPHEP-metal complexes showed poor abilities to separate homophenylalanine (Hphe) with α below 1.5. The influences of extraction temperature, concentrations of (S)-MeO-BIPHEP-metal complexes and pH of aqueous phase on extractions were systematically investigated. On the basis of single-factor experiments, the extraction conditions of separating Phe, Pheg, Nphe, Tyr and Cpheg were optimized by response surface methodology. After optimization, the maximum performance factors (pf) for Phe, Pheg, Nphe, Tyr and Cpheg were 0.11865, 0.13487, 0.17706, 0.21882 and 0.13579, respectively.

（S）-MeO-BIPHEP-金属配合物首先被用作手性萃取剂以分离氨基酸对映体。六个氨基酸对映体被用来评估（S）-MeO-BIPHEP-金属配合物的立体选择性。结果表明（S）-MeO-BIPHEP-Cu配合物具有良好的分离苯丙氨酸（Phe），苯基甘氨酸（Pheg），酪氨酸（Tyr）和3-氯-苯基甘氨酸（Cpheg）对映体的能力，且具有对映体选择性（α）分别为2.56、2.24、4.22和1.81。（S）-MeO-BIPHEP-Pd配合物表现出良好的分离4-硝基-苯丙氨酸（Nphe）的能力，操作对映选择性（α）为3.87。全部（S）-MeO-BIPHEP-金属络合物分离α值低于1.5的高苯丙氨酸（Hphe）的能力较弱。系统地研究了萃取温度，（S）-MeO-BIPHEP-金属配合物浓度和水相pH对萃取的影响。在单因素实验的基础上，采用响应面法优化了分离Phe，Pheg，Nphe，Tyr和Cpheg的提取条件。经过优化后，Phe，Pheg，Nphe，Tyr和Cpheg的最大性能因子（pf）分别为0.11865、0.13487、0.17706、0.21882和0.13579。