Abstract This study aims to examine the inhibitory effects of the bacteriocin CHQS, produced by Enterococcus faecalis TG2, and its mode of action against Listeria ivanovii DSMZ 20750T, which is one of the pathogens sensitive to CHQS. The minimum inhibitory concentration, minimum bactericide concentration and time-inhibition curves were measured to confirm the strong effects of CHQS on L. ivanovii DSMZ 20750T, and its antibacterial mode of action was studied by observing the changes in cell membrane permeability, integrity, and proton motive force. The minimum inhibitory concentration and minimum bactericide concentration of CHQS were 26.74 ng/mL and 107.30 ng/mL, respectively, demonstrating that CHQS exhibited significantly strong and rapid antibacterial efficacy towards L. ivanovii DSMZ 20750T. The results showed that CHQS led to the leakage of K+ and electrolytes, the massive release of ATP and UV-absorbing materials, and the collapse of the proton motive force. The micrographs of scanning electron and transmission electron microscopy demonstrate the severe cellular destruction and release of intracellular substances induced by bacteriocin CHQS. Collectively, this study reveals the antibacterial activity and mode of action of CHQS against L. ivanovii DSMZ 20750T and demonstrates the mode of action of the inhibition by bacteriocins.

中文翻译：

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细菌素CHQS的作用方式，一种由粪肠球菌TG2产生的高抗菌活性细菌素

摘要 本研究旨在检测由粪肠球菌TG2产生的细菌素CHQS的抑制作用及其对CHQS敏感病原体之一伊凡诺维李斯特菌DSMZ 20750T的作用方式。测定最低抑菌浓度、最低杀菌剂浓度和时间-抑菌曲线，证实CHQS对L. ivanovii DSMZ 20750T的强作用，并通过观察细胞膜通透性、完整性和质子的变化研究其抑菌作用方式动力。CHQS 的最低抑菌浓度和最低杀菌浓度分别为 26.74 ng/mL 和 107.30 ng/mL，表明 CHQS 对 L. ivanovii DSMZ 20750T 表现出显着强且快速的抗菌功效。结果表明，CHQS导致K+和电解质的泄漏，ATP和紫外线吸收材料的大量释放，以及质子动力的崩溃。扫描电子显微镜和透射电子显微镜的显微照片表明细菌素 CHQS 引起了严重的细胞破坏和细胞内物质的释放。总的来说，这项研究揭示了 CHQS 对 L. ivanovii DSMZ 20750T 的抗菌活性和作用方式，并证明了细菌素抑制的作用方式。