Mitochondria and glutathione (GSH) play crucial roles in human pathologies. To better understand the disease mechanisms, two-photon fluorescence probes responding to mitochondria and glutathione were urgently pursued. Here, a series of two-photon fluorescence probes (5a, 5b and 5c) were prepared, which could detect GSH as well as target mitochondria. The probes delivered high and rapid selectivity towards GSH among the various biothiols and qualitatively monitored the endogenous cellular GSH in real-time via a two-photon laser confocal fluorescence microscopy. Furthermore, the theoretical understanding of high selectivity in two-photon fluorescence probes were also performed. The combined results demonstrated that the probes would function as efficient tools in bio-imaging and biological diagnosis.

线粒体和谷胱甘肽（GSH）在人类病理中起着至关重要的作用。为了更好地了解疾病机理，迫切寻求响应线粒体和谷胱甘肽的双光子荧光探针。在这里，准备了一系列的双光子荧光探针（5a，5b和5c），可以检测GSH以及靶线粒体。这些探针对各种生物硫醇中的GSH具有高而快速的选择性，并通过实时定性监测内源性细胞内GSH。双光子激光共聚焦荧光显微镜。此外，还对双光子荧光探针中的高选择性进行了理论理解。结合的结果表明，这些探针将在生物成像和生物诊断中充当有效的工具。