The effect of dissolved oxygen fluctuations on suspended plant cells was investigated using a scale‐down stirred bioreactor system to simulate the heterogeneous conditions found in large‐scale vessels. Transgenic Nicotiana tabacum cells expressing IgG1 antibody and Thalictrum minus cells producing berberine were subjected to gas switching cycles based on a stochastic model of log‐normal circulation time distributions. Because pumping severely damaged the cells, the scale‐down approach was restricted to a single‐vessel system. Dissolved oxygen oscillations of amplitude about ±5% air saturation were achieved in the N. tabacum cultures; however, most simulations produced highly damped fluctuations due to oxygen transfer limitations and no significant effect on the cultures was observed. In contrast, berberine production by T. minus cells was reduced when fluctuations of ±10–15% air saturation were superimposed on mean dissolved oxygen tensions of 25 and 35% air saturation. This work demonstrates that dissolved oxygen gradients such as those found in large‐scale mixing vessels can be detrimental to plant cell cultures even when sufficient average dissolved oxygen levels are provided. © 2018 American Institute of Chemical Engineers AIChE J, 64: 4281–4288, 2018

中文翻译：

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悬浮植物细胞培养的生物反应器按比例缩小研究

使用按比例缩小的搅拌生物反应器系统研究了溶解氧波动对悬浮植物细胞的影响，以模拟在大型容器中发现的异质条件。根据对数正态循环时间分布的随机模型，将表达IgG1抗体的转基因烟草细胞和生产小ber碱的海藻减去细胞，进行气体切换循环。由于抽水严重破坏了细胞，因此按比例缩小方法仅限于单容器系统。在烟草中获得了大约为空气饱和度±5％的溶解氧振荡文化；然而，由于氧转移限制，大多数模拟产生了高度衰减的波动，并且未观察到对培养物的显着影响。相反，将空气饱和度的±10–15％波动叠加到空气饱和度25和35％的平均溶解氧张力上时，减短毛虫的小ber碱产量会降低。这项工作表明，即使提供了足够的平均溶解氧水平，溶解氧梯度（例如在大型混合容器中发现的那些）也可能有害于植物细胞培养。©2018美国化学工程师学会AIChE J，64：4281–4288，2018