Lipid vesicles are an attractive model membrane experimental platform that is widely used in a biological context. The stability of vesicles can affect their performance and depends on various experimental conditions. How bio-related ions affect vesicle morphology is poorly understood in some cases. Herein, we investigated changes in vesicle morphology influenced by cation in the static and flowing environments. The effects of different mono- and di-valent metal cations on the morphology of lipid vesicles were systematically studied using the various techniques. The results showed that divalent cations caused significant aggregation or fusion of lipid vesicles, but monovalent cations had little effect on the vesicle morphology. Cation binding increased the net surface potential of vesicles, leading to changes in the zeta potential. The same qualitative kinetics were observed for cations that had the same valence at the same ionic strength. However, different types of cations gave different quantitative effects. The order of the ability to destroy the vesicle morphology was Cu²⁺ > Mg²⁺ > Ca²⁺ > Na⁺ > K⁺. These results are of practical value in the use of lipid vesicles as a bionic model, and help to shed light on the role of ions at membrane surfaces and interfaces.

脂质囊泡是一种有吸引力的模型膜实验平台，广泛用于生物学环境。囊泡的稳定性会影响其性能，并取决于各种实验条件。在某些情况下，人们对与生物有关的离子如何影响囊泡形态的了解很少。在这里，我们研究了在静态和流动环境中阳离子对囊泡形态的影响。使用各种技术系统地研究了不同的单价和二价金属阳离子对脂质囊泡形态的影响。结果表明，二价阳离子引起脂质囊泡的显着聚集或融合，而单价阳离子对囊泡的形态影响很小。阳离子结合增加了囊泡的净表面电势，导致ζ电势的变化。对于在相同离子强度下具有相同化合价的阳离子，观察到相同的定性动力学。但是，不同类型的阳离子具有不同的定量作用。破坏囊泡形态的能力顺序是铜²⁺  >镁²⁺  >钙²⁺  >钠⁺  >钾⁺。这些结果在将脂质囊泡用作仿生模型中具有实用价值，并有助于阐明离子在膜表面和界面上的作用。