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Understanding the role of extracts from sea buckthorn seed residues in anti-melanogenesis properties on B16F10 melanoma cells†
Food & Function ( IF 5.1 ) Pub Date : 2018-09-24 00:00:00 , DOI: 10.1039/c8fo01427b
Jiachan Zhang 1, 2, 3, 4, 5 , Changtao Wang 4, 6, 7, 8 , Chengtao Wang 4, 5, 6, 7, 8 , Baoguo Sun 1, 2, 3, 4, 5 , Cai Qi 4, 9, 10, 11, 12
Affiliation  

The hydroalcoholic extract of sea buckthorn (Hippophae rhamnoides L.) seed residues (HYD-SBSR) is a potential skin whitening agent. To test this material as a potential skin whitening agent, we identified and quantified the main chemical constituents of HYD-SBSR by using ultra-performance liquid chromatography coupled with quadruple time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS) and ultra-high performance liquid chromatography coupled with triple quadruple mass spectrometry (UPLC-QQQ-MS). The anti-melanogenesis properties of HYD-SBSR on B16F10 mouse melanoma cells were analysed and the mechanism was measured on both the transcriptional and translational levels. About 24 compounds were identified. Kaempferol and its derivatives were the main compounds with a concentration of about (2796.22 ± 31.55) μg per g DW. The following order among the detected compounds was observed: quercetin and its derivatives > isorhamnetin and its derivatives > procyanidins. HYD-SBSR has a strong antioxidant activity but with a slight cytotoxic effect on B16F10 when treated with 45.45 μg mL−1 and 4.55 μg mL−1 respectively, for 48 h. HYD-SBSR has been found to significantly decrease melanin content (P < 0.01) in 24 h, 48 h, and 72 h. Additionally, strong inhibitory extracellular tyrosinase activities and decreasing intracellular tyrosinase activities were also observed (P < 0.01). HYD-SBSR shows inhibitory effects on the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TRP-1), and the secretion of TYR and TRP-1 proteins in cell lines. The protein levels of tyrosinase-related protein 2 (TRP-2) and microphthalmia-associated transcription factor (MITF) showed no significant difference. HYD-SBSR may inhibit melanin synthesis by decreasing the tyrosinase activity and down-regulating the expression of TYR and TRP-1 which were probably induced by other transcriptional factors rather than MITF.

中文翻译:

了解沙棘种子残留物中的提取物在B16F10黑色素瘤细胞的抗黑色素生成特性中的作用

沙棘(沙棘)的水醇提取物。L.)种子残留物(HYD-SBSR)是潜在的皮肤增白剂。为了测试这种材料是否是潜在的皮肤增白剂,我们通过使用超高效液相色谱与四倍飞行时间串联质谱(UPLC-QTOF-MS / MS)鉴定并量化了HYD-SBSR的主要化学成分。超高效液相色谱与三重四极杆质谱联用(UPLC-QQQ-MS)。分析了HYD-SBSR对B16F10小鼠黑素瘤细胞的抗黑色素生成特性,并在转录和翻译水平上研究了该机理。鉴定出约24种化合物。山emp酚及其衍生物是主要化合物,每克DW的浓度约为(2796.22±31.55)μg。在检测到的化合物中,观察到以下顺序:槲皮素及其衍生物>异鼠李素及其衍生物>原花青素。HYD-SBSR具有很强的抗氧化活性,但用45.45μgmL处理后对B16F10具有轻微的细胞毒性作用-1和4.55μgmL -1持续48小时。已发现HYD-SBSR在24 h,48 h和72 h中可显着降低黑色素含量( P <0.01)。此外,还观察到强烈的抑制性细胞外酪氨酸酶活性和降低的细胞内酪氨酸酶活性( P<0.01)。HYD-SBSR对酪氨酸酶(TYR)和酪氨酸酶相关蛋白1(TRP-1)的表达以及细胞系中TYR和TRP-1蛋白的分泌具有抑制作用。酪氨酸酶相关蛋白2(TRP-2)和小眼症相关转录因子(MITF)的蛋白水平无显着差异。HYD-SBSR可能通过降低酪氨酸酶活性并下调TYR和TRP-1的表达来抑制黑色素的合成,而TYR和TRP-1的表达可能是由其他转录因子而非MITF诱导的。
更新日期:2018-09-24
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