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Imaging and Quantitative Detection of Lipid Droplets by Yellow Fluorescent Probes in Liver Sections of Plasmodium Infected Mice and Third Stage Human Cervical Cancer Tissues
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2018-09-24 00:00:00 , DOI: 10.1021/acs.bioconjchem.8b00552 Ashutosh Sharma 1 , Ajay K. Jha 1 , Shachi Mishra 1 , Ankita Jain 2 , Bhavana S. Chauhan 3 , Manoj Kathuria 4 , Kundan S. Rawat 1, 5 , Neeraj M. Gupta 1 , Renu Tripathi 3 , Kalyan Mitra 4 , Monika Sachdev 2 , Madan L. B. Bhatt 6 , Atul Goel 1, 5
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2018-09-24 00:00:00 , DOI: 10.1021/acs.bioconjchem.8b00552 Ashutosh Sharma 1 , Ajay K. Jha 1 , Shachi Mishra 1 , Ankita Jain 2 , Bhavana S. Chauhan 3 , Manoj Kathuria 4 , Kundan S. Rawat 1, 5 , Neeraj M. Gupta 1 , Renu Tripathi 3 , Kalyan Mitra 4 , Monika Sachdev 2 , Madan L. B. Bhatt 6 , Atul Goel 1, 5
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The diagnosis and prognosis of the disease associated with lipid irregularity are areas of extreme significance. In this direction, fluoranthene based yellow fluorescent probes (FLUN-550, FLUN-552, FLUN-547) were designed and synthesized by conjugating the ethanolamine headgroup of the phospholipid phosphatidyl-ethanolamine present in biological membranes. Owing to unique photophysical properties and aqueous compatibility, these probes were successfully employed for staining lipid droplets (LDs) in preadipocytes and Leishmania donovani promastigotes. Furthermore, using the fluorescent probes FLUN-550 and FLUN-552 we successfully imaged and quantitatively detected the excess accumulation of lipids in a liver section of Plasmodium yoelii MDR infected mice (3- to 4-fold) and the tissue sections of third stage human cervical cancer patients (1.5- to 2-fold) compared to normal tissues. To the best of our knowledge, this is the first report of yellow fluorescent probes for imaging and quantitative detection of LDs in human cervical cancer tissues. These new yellow fluorescent lipid probes (FLUN-550 and FLUN-552) showed great potential for diagnosis of cervical cancer patients.
中文翻译：
黄色荧光探针在疟原虫感染的小鼠和第三阶段人宫颈癌组织的肝脏切片中对血滴的成像和定量检测
与脂质不规则有关的疾病的诊断和预后是极为重要的领域。在这个方向上，通过缀合生物膜中存在的磷脂磷脂酰乙醇胺的乙醇胺头基，设计并合成了基于荧蒽的黄色荧光探针（FLUN-550，FLUN-552，FLUN-547）。由于独特的光物理特性和水相容性，这些探针已成功用于染色前脂肪细胞和多形利什曼原虫前鞭毛体中的脂质滴（LDs）。此外，使用荧光探针FLUN-550和FLUN-552我们成功地成像并定量检测了约氏疟原虫MDR感染小鼠肝脏切片（3-4倍）和第三期宫颈癌患者组织切片（1.5-2倍）中脂质的过量积聚。正常组织。据我们所知，这是黄色荧光探针用于人类宫颈癌组织中LDs的成像和定量检测的首次报道。这些新的黄色荧光脂质探针（FLUN-550和FLUN-552）在诊断宫颈癌患者中显示出巨大的潜力。
更新日期：2018-09-24
中文翻译：
黄色荧光探针在疟原虫感染的小鼠和第三阶段人宫颈癌组织的肝脏切片中对血滴的成像和定量检测
与脂质不规则有关的疾病的诊断和预后是极为重要的领域。在这个方向上，通过缀合生物膜中存在的磷脂磷脂酰乙醇胺的乙醇胺头基，设计并合成了基于荧蒽的黄色荧光探针（FLUN-550，FLUN-552，FLUN-547）。由于独特的光物理特性和水相容性，这些探针已成功用于染色前脂肪细胞和多形利什曼原虫前鞭毛体中的脂质滴（LDs）。此外，使用荧光探针FLUN-550和FLUN-552我们成功地成像并定量检测了约氏疟原虫MDR感染小鼠肝脏切片（3-4倍）和第三期宫颈癌患者组织切片（1.5-2倍）中脂质的过量积聚。正常组织。据我们所知，这是黄色荧光探针用于人类宫颈癌组织中LDs的成像和定量检测的首次报道。这些新的黄色荧光脂质探针（FLUN-550和FLUN-552）在诊断宫颈癌患者中显示出巨大的潜力。
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