In the present study, a convenient and highly efficient method was developed to quantify aflatoxin B₁ (AFB₁) in oil samples. Low temperature clean-up (LTC) followed by immuno magnetic solid phase extraction (IMSPE) was used to clean up oil samples. LTC assisted in freezing out the interference from the oil matrix while IMSPE further helped to preconcentrate the targeted analyte. For IMSPE, we synthesised and characterised anti-AFB₁ monoclonal antibody-functionalised magnetic nanoparticles. Oil sample extraction was then carried out using LTC-IMSPE. The proposed method showed satisfactory efficiency and reproducibility with recovery rates being within the range of 79.6–117.9%, with a relative standard deviation below 11.48%. The sensitivity of the method was satisfactory with the limits of detection and quantification being as low as 0.0048 and 0.0126 ng·g⁻¹, respectively. Real sample analysis was carried out for five kinds of different vegetable oils. The results suggest that the method developed is very sensitive and accurate.

在本研究中，开发了一种方便高效的方法来定量油样中的黄曲霉毒素B ₁（AFB ₁）。使用低温净化（LTC），然后进行免疫磁固相萃取（IMSPE）净化油样。LTC帮助冻结了油基质中的干扰，而IMSPE进一步帮助了对目标分析物的预浓缩。对于IMSPE，我们合成并鉴定了抗AFB ₁单克隆抗体功能化的磁性纳米粒子。然后使用LTC-IMSPE进行油样提取。所提出的方法显示出令人满意的效率和可重复性，回收率在79.6-117.9％的范围内，相对标准偏差低于11.48％。该方法的灵敏度令人满意，检测和定量限分别低至0.0048和0.0126 ng·g ^-1。对五种不同的植物油进行了实际样品分析。结果表明所开发的方法非常灵敏和准确。