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A ratiometric fluorescent probe for the detection of peroxynitrite with simple synthesis and large emission shift and its application in cells image
Dyes and Pigments ( IF 4.1 ) Pub Date : 2018-09-24 , DOI: 10.1016/j.dyepig.2018.09.059
Dong-Ye Zhou , Juan Ou-Yang , Yongfei Li , Wen-Li Jiang , Yang Tian , Zheng-Ming Yi , Chun-Yan Li

Peroxynitrite (ONOO) is an important kind of reactive oxygen species (ROS), played an essential role in physiology and pathology. To date, various fluorescent probes for ONOO have been constructed successfully, but these probes exhibit turn-on fluorescence signal in single channel. While, ratiometric fluorescent probes, especially those probes with large emission shift, are suitable for bioimage in two channels. Herein, a hemicyanine-based fluorescent probe named Cy-NH2 was designed and synthesized for ratiometric detection of ONOO. The characteristics of Cy-NH2 are as follows: (1) The probe possesses a simple structure, which can be obtained by easy synthetic steps; (2) The ratiometric fluorescent probe owns large emission shift (248 nm) due to the disruption of π-conjugation system caused by strong oxidizing ability of ONOO; (3) The probe exhibits high sensitivity to ONOO with 1728-fold enhancement of fluorescence intensity ratio and superior selectivity for ONOO over various potentially interfering biological analytes; (4) The ratiometric fluorescent probe is applied for the image detection of exogenous and endogenous ONOO in living cells successfully.



中文翻译:

一种合成简单,发射位移大的过氧亚硝酸根比例荧光探针及其在细胞图像中的应用

过氧亚硝酸盐(ONOO - )是一种重要的活性氧(ROS),在生理和病理方面发挥了重要作用。迄今为止,各种ONOO荧光探针-已经成功地构建,但这些探针表现出导通在单一信道的荧光信号。同时,比例荧光探针,尤其是那些具有较大发射位移的探针,适合在两个通道中进行生物成像。在本文中,命名为CY-NH基于半花青荧光探针2被设计和用于ONOO的比率检测合成- 。Cy-NH 2的特征如下:(1)该探针结构简单,可以通过简单的合成步骤得到。(2)比率荧光探针拥有大的发射位移(248纳米)由于由ONOO的强氧化能力π共轭系统的破坏- ; (3)ONOO的探针表现出高的灵敏度-用的荧光强度比和ONOO优良的选择性1728倍的增强-在各种潜在干扰生物分析物; (4)比率荧光探针被应用于外源性和内源性ONOO的图像检测-在活细胞中成功。

更新日期:2018-09-24
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