A pH-independent and water soluble fluorescent probe based on a benzothiazole moiety was prepared for the detection of nitroreductase under biological conditions. Upon the addition of nitroreductase in the presence of NADH, the probe displayed both a colorless-to-yellow optical change and a fluorescence turn-on response in the visible region, with a very low limit of detection of the enzyme (LOD = 9.8 ng/mL) in PBS buffer. Due to its hydroxyethyl tethering group, the probe was highly soluble in water and exhibited the characteristic optical response in a wide range of pH values. Further cellular application was successfully performed and clear mitochondrial images were obtained with A549 cells.

制备了基于苯并噻唑部分的非pH依赖性且水溶性的荧光探针，用于在生物条件下检测硝基还原酶。在存在NADH的情况下添加硝基还原酶后，该探针在可见光区域显示无色至黄色的光学变化和荧光开启响应，并且酶的检出限非常低（LOD = 9.8 ng / mL）在PBS缓冲液中。由于其羟乙基束缚基团，该探针高度溶于水，并在很宽的pH值范围内表现出独特的光学响应。成功进行了进一步的细胞应用，并用A549细胞获得了清晰的线粒体图像。