Sugar alcohols (polyols) exist widely in nature. While some specific sugar alcohol phosphatases are known, there is no known phosphatase for some important sugar alcohols (e.g., sorbitol-6-phosphate). Using liquid chromatography–mass spectrometry-based metabolomics, we screened yeast strains with putative phosphatases of unknown function deleted. We show that the yeast gene YNL010W, which has close homologues in all fungi species and some plants, encodes a sugar alcohol phosphatase. We term this enzyme, which hydrolyzes sorbitol-6-phosphate, ribitol-5-phosphate, and (d)-glycerol-3-phosphate, polyol phosphatase 1 or PYP1. Polyol phosphates are structural analogs of the enediol intermediate of phosphoglucose isomerase (Pgi). We find that sorbitol-6-phosphate and ribitol-5-phosphate inhibit Pgi and that Pyp1 activity is important for yeast to maintain Pgi activity in the presence of environmental sugar alcohols. Pyp1 expression is strongly positively correlated with yeast growth rate, presumably because faster growth requires greater glycolytic and accordingly Pgi flux. Thus, yeast express the previously uncharacterized enzyme Pyp1 to prevent inhibition of glycolysis by sugar alcohol phosphates. Pyp1 may be useful for engineering sugar alcohol production.

中文翻译：

---

酵母糖醇磷酸酶的发现和功能表征

糖醇（多元醇）在自然界广泛存在。尽管某些特定的糖醇磷酸酶是已知的，但对于某些重要的糖醇（例如，山梨糖醇6-磷酸酯）尚无已知的磷酸酶。使用基于液相色谱-质谱的代谢组学，我们筛选了具有未知功能的假定磷酸酶缺失的酵母菌株。我们显示酵母基因YNL010W，在所有真菌物种和某些植物中都有紧密的同源性，编码糖醇磷酸酶。我们术语此酶，它水解山梨糖醇-6-磷酸，核糖醇-5-磷酸，和（d） -甘油-3-磷酸，p醇ÿ醇p hosphatase 1或PYP1。多元醇磷酸酯是磷酸葡萄糖异构酶（Pgi）的烯二醇中间体的结构类似物。我们发现，山梨糖醇6-磷酸和核糖醇5-磷酸抑制Pgi，并且Pyp1活性对于在环境糖醇存在下维持Pgi活性的酵母很重要。Pyp1的表达与酵母的生长速度呈正相关，大概是因为更快的生长需要更大的糖酵解和相应的Pgi通量。因此，酵母表达以前未表征的酶Pyp1，以防止糖醇磷酸盐抑制糖酵解。Pyp1可能对工程糖醇生产有用。